ArticleThe Journal of molecular diagnostics : JMD2006
An oligonucleotide microarray for high-throughput sequencing of the mitochondrial genome.
Article in The Journal of molecular diagnostics : JMD, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 77 citations in OpenAlex.
- Epidermal nerve fiber density, oxidative stress, and mitochondrial haplogroups in HIV-infected Thais initiating therapy.AIDS (London, England) · 2014Trial
- Mitochondrial genome study in blood of maternally inherited ALS cases.Human genomics · 2023Article
- Associations of Peripheral Artery Disease With Calf Skeletal Muscle Mitochondrial DNA Heteroplasmy.Journal of the American Heart Association · 2020Article
- Mitochondrial DNA Mutations in Grade II and III Glioma Cell Lines Are Associated with Significant Mitochondrial Dysfunction and Higher Oxidative Stress.Frontiers in physiology · 2017Article
- Diagnosis and management of mitochondrial disease: a consensus statement from the Mitochondrial Medicine Society.Genetics in medicine : official journal of the American College of Medical Genetics · 2015Review
- Inosine triphosphate pyrophosphohydrolase (ITPA) polymorphic sequence variants in adult hematological malignancy patients and possible association with mitochondrial DNA defects.Journal of hematology & oncology · 2013Article
- Detection of mitochondrial deoxyribonucleic acid alterations in urine from urothelial cell carcinoma patients.International journal of cancer · 2012Article
- Mitochondrial DNA mutations in respiratory complex-I in never-smoker lung cancer patients contribute to lung cancer progression and associated with EGFR gene mutation.Journal of cellular physiology · 2012Article
- Insights into N-calls of mitochondrial DNA sequencing using MitoChip v2.0.BMC research notes · 2011Article
- Mitochondrial genome sequence analysis: a custom bioinformatics pipeline substantially improves Affymetrix MitoChip v2.0 call rate and accuracy.BMC bioinformatics · 2011Article
- Metabolic alterations in cancer cells and therapeutic implications.Chinese journal of cancer · 2011Review
- A new phenotype of mitochondrial disease characterized by familial late-onset predominant axial myopathy and encephalopathy.Acta neuropathologica · 2011Article
- Mitochondrial subversion in cancer.Cancer prevention research (Philadelphia, Pa.) · 2011Review
- Mitochondrial DNA mutations in head and neck cancer are infrequent and lack prognostic utility.British journal of cancer · 2011Article
- Mitochondrial-nuclear epistasis: implications for human aging and longevity.Ageing research reviews · 2011Review
- Fluorescent xDNA nucleotides as efficient substrates for a template-independent polymerase.Nucleic acids research · 2011Article
- Evaluation of second-generation sequencing of 19 dilated cardiomyopathy genes for clinical applications.The Journal of molecular diagnostics : JMD · 2010Article
- Mitochondrial DNA mutation in normal margins and tumors of recurrent head and neck squamous cell carcinoma patients.Cancer prevention research (Philadelphia, Pa.) · 2010Article
- Article
- A novel custom resequencing array for dilated cardiomyopathy.Genetics in medicine : official journal of the American College of Medical Genetics · 2010Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Previously we developed an oligonucleotide sequencing microarray (MitoChip) as an array-based sequencing platform for rapid and high-throughput analysis of mitochondrial DNA. The first generation MitoChip, however, was not tiled with probes for the noncoding D-loop region, a site frequently mutated in human cancers. Here we report the development of a second-generation MitoChip (v2.0) with oligonucleotide probes to sequence the entire mitochondrial genome. In addition, the MitoChip v2.0 contains redundant tiling of sequences for 500 of the most common haplotypes including single-nucleotide changes, insertions, and deletions. Sequencing results from 14 primary head and neck tumor tissues demonstrated that the v2.0 MitoChips detected a larger number of variants than the original version. Multiple coding region variants detected only in the second generation MitoChips, but not the earlier chip version, were further confirmed with conventional sequencing. Moreover, 31 variations in noncoding region were identified using MitoChips v2.0. Replicate experiments demonstrated >99.99% reproducibility in the second generation MitoChip. In seven head and neck cancer samples with matched lymphocyte DNA, the MitoChip v2.0 detected at least one cancer-associated mitochondrial mutation in four (57%) samples. These results indicate that the second generation MitoChip is a high-throughput platform for identification of mitochondrial DNA mutations in primary tumors.
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Registered trials
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