ArticleMolecular and cellular biology1990
Many transcribed regions of the Onchocerca volvulus genome contain the spliced leader sequence of Caenorhabditis elegans.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 47 citations in OpenAlex.
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- Heartworm (Dirofilaria immitis) infection in a leopard (Panthera pardus pardus) housed in a zoological park in north-eastern Italy.Parasites & vectors · 2010Article
- Characterization of the manganese superoxide dismutase cDNA and gene from the human parasite Onchocerca volvulus.The Biochemical journal · 1995Article
- Orphon spliced-leader sequences form part of a repetitive element in Angiostrongylus cantonensis.Nucleic acids research · 1995Article
- Epitopes of the Onchocerca volvulus RAL1 antigen, a member of the calreticulin family of proteins, recognized by sera from patients with onchocerciasis.Infection and immunity · 1994Article
- Functional analysis of a C. elegans trans-splice acceptor.Nucleic acids research · 1993Article
- Trypanosoma vivax: linkage of the mini-exon (spliced leader) and 5S ribosomal RNA genes.Nucleic acids research · 1992Article
- In Euglena, spliced-leader RNA (SL-RNA) and 5S rRNA genes are tandemly repeated.Nucleic acids research · 1992Article
- Intramolecular base pairing between the nematode spliced leader and its 5' splice site is not essential for trans-splicing in vitro.The EMBO journal · 1991Article
- Insertion of part of an intron into the 5' untranslated region of a Caenorhabditis elegans gene converts it into a trans-spliced gene.Molecular and cellular biology · 1991Article
- New nucleotide sequence data on the EMBL file server.Nucleic acids research · 1990Article
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3 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Genomic DNAs of the related parasitic nematodes Onchocerca volvulus and Dirofilariae immitis, and a cDNA library of O. volvulus, were examined for the presence of the 22-nucleotide spliced leader (SL) found at the 5' ends of 10 to 15% of the mRNAs in the free-living nematode Caenorhabditis elegans. As in C. elegans, genes for the SL RNA are linked to the repetitive 5S rRNA genes of O. volvulus and D. immitis, but unlike C. elegans, they are in the same orientation as the 5S rRNA genes within the repeat unit. In O. volvulus the SL sequence is also encoded at more than 30 additional genomic locations and occurs at interior sites within many transcripts. Sequence determinations of four different cDNAs of O. volvulus, each containing an internal copy of the SL within a conserved 25mer, and one corresponding genomic DNA clone indicate that this sequence is not trans spliced onto these RNAs, but is encoded within the genes. The RNAs of two of these cDNAs appear to be developmentally regulated, since they occur in adult O. volvulus but were not detected in the infective L3 stage larvae. In contrast, actin mRNAs are present at all developmental stages, and at least one actin mRNA species contains a trans-spliced 5' SL. The internal locations of the SL in various transcripts and its perfect sequence conservation among parasitic and free-living nematodes argues that it serves specific, and perhaps multiple, functions for these organisms.
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