ArticleJournal of anatomy2006
Morphological changes of isolated rat pancreatic islets: a structural, ultrastructural and morphometric study.
Article in Journal of anatomy, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 47 citations in OpenAlex.
- The role of lutein-rich purple sweet potato leaf extract on the amelioration of diabetic retinopathy in streptozotocin-induced Sprague-Dawley rats.Frontiers in pharmacology · 2023Article
- Review
- Pancreas Preservation with a Neutrophil Elastase Inhibitor, Alvelestat, Contributes to Improvement of Porcine Islet Isolation and Transplantation.Journal of clinical medicine · 2022Article
- Physiological and Histological Changes in Pancreatic Gland Associated with Ageing in Local Rabbits in Iraq.Archives of Razi Institute · 2021Article
- Recruited fibroblasts reconstitute the peri-islet membrane: a longitudinal imaging study of human islet grafting and revascularisation.Diabetologia · 2020Article
- Endocrine cell type sorting and mature architecture in the islets of Langerhans require expression of Roundabout receptors in β cells.Scientific reports · 2018Article
- Enzyme Development for Human Islet Isolation: Five Decades of Progress or Stagnation?The review of diabetic studies : RDS · 2017Review
- An optimized protocol for purification of functional islets of Langerhans.Laboratory investigation; a journal of technical methods and pathology · 2017Article
- Imaging of Hydrogel Microsphere Structure and Foreign Body Response Based on Endogenous X-Ray Phase Contrast.Tissue engineering. Part C, Methods · 2016Article
- Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability.Journal of diabetes research · 2016Article
- Morphological changes of post-isolation of caprine pancreatic islet.In vitro cellular & developmental biology. Animal · 2015Article
- Impact of islet size on pancreatic islet transplantation and potential interventions to improve outcome.Cell transplantation · 2015Article
- Article
- Co-transplantation of mesenchymal stem cells maintains islet organisation and morphology in mice.Diabetologia · 2011Article
- Islet-like cell aggregates generated from human adipose tissue derived stem cells ameliorate experimental diabetes in mice.PloS one · 2011Article
- FEM-based oxygen consumption and cell viability models for avascular pancreatic islets.Theoretical biology & medical modelling · 2009Article
- Revascularization and remodelling of pancreatic islets grafted under the kidney capsule.Journal of anatomy · 2007Article
- Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Improved techniques for pancreatic islet extraction can yield a reasonable number of transplantable cells. However, the isolation and purification process may damage the islets and impair their physiological functions. The aim of this study was to determine the effect of the isolation procedure on the structure of isolated islets and to correlate this with their functionality. Islets were isolated from rat pancreata and purified by Eurocollins-Ficoll discontinuous density gradient processing, and then processed for light microscopy, and scanning and transmission electron microscopy. Morphometric analysis was also performed. Islet functionality was determined by reversal of streptozotocin-induced diabetes and the intraperitoneal glucose tolerance test in a syngeneic rat model of pancreatic islet transplantation. Fragments of variable size and shape comprised a relatively large proportion (26%) of the isolated endocrine tissue. Isolated islets showed slight alterations of cell ultrastructure. Major damage (including breakage of the plasma membrane) and loss of cells were observed in the peripheral cells of the isolated islets. An equal mass of islet equivalent (IEq, islets with an average diameter of 150 microm), but with a different islet equivalent/islet number ratio, was transplanted in diabetic animals. When larger and more complete islets were transplanted (higher ratio), better function of the graft was observed by reversal of hyperglycaemia and response to the glucose tolerance test as compared with the functionality and response of smaller (fragmented) islets transplanted (lower ratio). Digestion, trauma and hypoxia during isolation are responsible for qualitative and quantitative changes of isolated islets. Alterations in normal secretory function after the transplant were related to lower islet equivalent/islet number ratio. The incomplete integrity of the islets may explain the failure of the fine glycaemic metabolic regulation.
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Registered trials
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