ArticleBMC biochemistry2006
The DNA polymerase activity of Pol epsilon holoenzyme is required for rapid and efficient chromosomal DNA replication in Xenopus egg extracts.
Article in BMC biochemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- The non-catalytic role of DNA polymerase epsilon in replication initiation in human cells.Nature communications · 2022Article
- The Sulfolobus solfataricus GINS Complex Stimulates DNA Binding and Processive DNA Unwinding by Minichromosome Maintenance Helicase.Journal of bacteriology · 2015Article
- Comparison of the kinetic parameters of the truncated catalytic subunit and holoenzyme of human DNA polymerase ɛ.DNA repair · 2015Article
- Article
- Polymerase ε1 mutation in a human syndrome with facial dysmorphism, immunodeficiency, livedo, and short stature ("FILS syndrome").The Journal of experimental medicine · 2012Article
- The C-terminus of Dpb2 is required for interaction with Pol2 and for cell viability.Nucleic acids research · 2012Article
- Segregation of replicative DNA polymerases during S phase: DNA polymerase ε, but not DNA polymerases α/δ, are associated with lamins throughout S phase in human cells.The Journal of biological chemistry · 2012Article
- DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.Molecular biology of the cell · 2012Article
- Studies on human DNA polymerase epsilon and GINS complex and their role in DNA replication.The Journal of biological chemistry · 2011Article
- Identification of genes differentially expressed by calorie restriction in the rotifer (Brachionus plicatilis).Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2010Article
- Reduced expression of GINS complex members induces hallmarks of pre-malignancy in primary untransformed human cells.Cell cycle (Georgetown, Tex.) · 2009Article
- SV40 DNA replication: from the A gene to a nanomachine.Virology · 2009Review
- DNA polymerase epsilon: a polymerase of unusual size (and complexity).Progress in nucleic acid research and molecular biology · 2008Review
- Crystal structure of the human GINS complex.Genes & development · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDNA polymerase epsilon (Pol epsilon) is involved in DNA replication, repair, and cell-cycle checkpoint control in eukaryotic cells. Although the roles of replicative Pol alpha and Pol delta in chromosomal DNA replication are relatively well understood and well documented, the precise role of Pol epsilon in chromosomal DNA replication is not well understood.
resultsThis study uses a Xenopus egg extract DNA replication system to further elucidate the replicative role(s) played by Pol epsilon. Previous studies show that the initiation timing and elongation of chromosomal DNA replication are markedly impaired in Pol epsilon-depleted Xenopus egg extracts, with reduced accumulation of replicative intermediates and products. This study shows that normal replication is restored by addition of Pol epsilon holoenzyme to Pol epsilon-depleted extracts, but not by addition of polymerase-deficient forms of Pol epsilon, including polymerase point or deletion mutants or incomplete enzyme complexes. Evidence is also provided that Pol epsilon holoenzyme interacts directly with GINS, Cdc45p and Cut5p, each of which plays an important role in initiation of chromosomal DNA replication in eukaryotic cells.
conclusionThese results indicate that the DNA polymerase activity of Pol epsilon holoenzyme plays an essential role in normal chromosomal DNA replication in Xenopus egg extracts. These are the first biochemical data to show the DNA polymerase activity of Pol epsilon holoenzyme is essential for chromosomal DNA replication in higher eukaryotes, unlike in yeasts.
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