ArticleCancer science2006
Optimized ultrasound-mediated gene transfection in cancer cells.
Article in Cancer science, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis.Pharmaceutics · 2022Review
- Biostatistics of VHL-Gene Transfection in the Health Informatics Analysis of Renal Cell Carcinoma.Computational and mathematical methods in medicine · 2022Article
- Article
- Ultrasound-induced molecular delivery to erythrocytes using a microfluidic system.Biomicrofluidics · 2020Article
- A review of low-intensity ultrasound for cancer therapy.Ultrasound in medicine & biology · 2015Review
- Improving ultrasound gene transfection efficiency by controlling ultrasound excitation of microbubbles.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- Augmentation of transgenic expression by ultrasound‑mediated liposome microbubble destruction.Molecular medicine reports · 2012Article
- New chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization.International journal of nanomedicine · 2012Article
- Sonoporation delivery of interleukin-27 gene therapy efficiently reduces prostate tumor cell growth in vivo.Human gene therapy · 2011Article
- Targeted gene transfection from microbubbles into vascular smooth muscle cells using focused, ultrasound-mediated delivery.Ultrasound in medicine & biology · 2010Article
- Hypotonia-induced cell swelling enhances ultrasound-induced mechanical damage to cancer cells.Journal of medical ultrasonics (2001) · 2010Article
- Growth inhibition of neurofibroma by ultrasound-mediated interferon γ transfection.Journal of medical ultrasonics (2001) · 2009Article
- Spatiotemporal effects of sonoporation measured by real-time calcium imaging.Ultrasound in medicine & biology · 2009Article
- Therapeutic potential of low-intensity ultrasound (part 2): biomolecular effects, sonotransfection, and sonopermeabilization.Journal of medical ultrasonics (2001) · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ultrasound-mediated gene transfection (sonotransfection) is a promising physical method for gene therapy, especially for cancer gene therapy. To investigate the optimal sonotransfection conditions and to determine whether the optimal transfection rate using sonotransfection is comparable to that of electrotransfection or liposome-mediated transfection, we sonicated different cancer cell lines (U937, HeLa, PC-3, Meth A and T-24) using a 1-MHz unfocused ultrasound at different intensities, pulse repetition frequencies and exposure times. The ideal ultrasound conditions were noted to be at 1.5 Watt/cm(2) pulsed at 0.5 Hz with a duty factor of 50%. The results showed that transfection rate increased with the number of pulses, and peaked between 10 and 15 pulses before it started to decline. Using such optimal conditions, we have shown that sonotransfection is superior to electrotransfection and liposome-mediated transfection at the fixed conditions used in the present study. These findings suggest that sonotransfection could be a better alternative to other non-viral methods (e.g. electroporation and liposome-mediated transfection) of gene transfection, particularly in cancer gene therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.