ArticlePlanta2007
Functional characterization of Nicotiana benthamiana homologs of peanut water deficit-induced genes by virus-induced gene silencing.
Article in Planta, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 57 citations in OpenAlex.
- Introgression of ΔTransgenic research · 2024Article
- Validation of molecular response of tuberization in response to elevated temperature by using a transient Virus Induced Gene Silencing (VIGS) in potato.Functional & integrative genomics · 2021Article
- Central role of 70-kDa heat shock protein in adaptation of plants to drought stress.Cell stress & chaperones · 2020Article
- ShNPSN11, a vesicle-transport-related gene, confers disease resistance in tomato to Oidium neolycopersici.The Biochemical journal · 2020Article
- Article
- Nitrogen stress-induced alterations in the leaf proteome of two wheat varieties grown at different nitrogen levels.Physiology and molecular biology of plants : an international journal of functional plant biology · 2015Article
- Assessing the tobacco-rattle-virus-based vectors system as an efficient gene silencing technique in Datura stramonium (Solanaceae).Virus genes · 2014Article
- Tobacco rattle virus-based virus-induced gene silencing in Nicotiana benthamiana.Nature protocols · 2014Article
- Virus-induced gene silencing is a versatile tool for unraveling the functional relevance of multiple abiotic-stress-responsive genes in crop plants.Frontiers in plant science · 2014Review
- A high-throughput virus-induced gene silencing protocol identifies genes involved in multi-stress tolerance.BMC plant biology · 2013Article
- Transgenic accumulation of a defective cucumber mosaic virus (CMV) replicase derived double stranded RNA modulates plant defence against CMV strains O and Y in potato.Transgenic research · 2013Article
- Characteristic of the pepper CaRGA2 gene in defense responses against Phytophthora capsici Leonian.International journal of molecular sciences · 2013Article
- Co-expression of AtbHLH17 and AtWRKY28 confers resistance to abiotic stress in Arabidopsis.Transgenic research · 2013Article
- AtCYP710A1 gene-mediated stigmasterol production plays a role in imparting temperature stress tolerance in Arabidopsis thaliana.Plant signaling & behavior · 2013Article
- A virus-induced gene silencing screen identifies a role for Thylakoid Formation1 in Pseudomonas syringae pv tomato symptom development in tomato and Arabidopsis.Plant physiology · 2010Article
- Identification and functional validation of a unique set of drought induced genes preferentially expressed in response to gradual water stress in peanut.Molecular genetics and genomics : MGG · 2009Article
- Influence of cytoplasmic heat shock protein 70 on viral infection of Nicotiana benthamiana.Molecular plant pathology · 2008Article
- Virus-induced gene silencing for functional analysis of selected genes.Plant cell reports · 2008Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Determining the functional role of genes that are differentially regulated during a stress response is challenging. In this study, few water deficit-induced genes from peanut were characterized in Nicotiana benthamiana using virus-induced gene silencing (VIGS) and their relevance for stress adaptation was validated. Twenty-five cDNA clones from peanut water deficit stress-induced cDNA library that had more than 50% nucleotide similarity with N. benthamiana or tomato homologs were selected. VIGS in peanut is not yet feasible and therefore we characterized these 25 genes in N. benthamiana. Increased membrane damage was seen under water deficit stress in most of the silenced plants signifying that many of these stress-induced genes are important to confer drought tolerance. Among the genes tested, silencing by homolog of flavonol 3-O-glucosyltransferase (F3OGT), homolog of alcohol dehydrogenase, homologous to salt inducible protein, and homolog of heat shock protein 70 showed more visible wilting symptoms compared with the control plants during water deficit stress. Interestingly, down-regulation of two genes, homologous to aspartic proteinase 2, and homolog of Jumonji class of transcription factor showed relative drought tolerant phenotypes. F3OGT silenced plants showed more wilting symptoms, membrane damage and chlorophyll degradation than any other silenced plants during water deficit. Our results demonstrate that VIGS approach can be used to characterize and assess the functional relevance of water deficit stress-induced cDNAs in a heterologous species.
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