ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2006
Chromosome-specific DNA repeat probes.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- An Incidental Finding of Gain of a Diminished Chromosome 12 Centromere in an Individual with Lymphocytosis: A Case Report and Clinical Implications in Cytogenetic Testing.Diagnostics (Basel, Switzerland) · 2025Article
- Generation of Dual-Color FISH probes targeting 9p21, Xp21, and 17p13.1 loci as diagnostic markers for some genetic disorders and cancer in Egypt.Journal, genetic engineering & biotechnology · 2025Article
- Cellular and genomic approaches for exploring structural chromosomal rearrangements.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Review
- Full Karyotype Interphase Cell Analysis.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2018Article
- Article
- High Performance DNA Probes for Perinatal Detection of Numerical Chromosome Aberrations.Advanced techniques in biology & medicine · 2015Article
- Bioinformatics tools allow targeted selection of chromosome enumeration probes and aneuploidy detection.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Article
- Chromosome-specific DNA repeats: rapid identification in silico and validation using fluorescence in situ hybridization.International journal of molecular sciences · 2012Article
- Bioinformatic Tools Identify Chromosome-Specific DNA Probes and Facilitate Risk Assessment by Detecting Aneusomies in Extra-embryonic Tissues.Current genomics · 2012Article
- Somatic genomic variations in extra-embryonic tissues.Current genomics · 2010Article
- Human interphase chromosomes: a review of available molecular cytogenetic technologies.Molecular cytogenetics · 2010Article
- DNA probe pooling for rapid delineation of chromosomal breakpoints.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- Rapid mapping of chromosomal breakpoints: from blood to BAC in 20 days.Folia histochemica et cytobiologica · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
In research as well as in clinical applications, fluorescence in situ hybridization (FISH) has gained increasing popularity as a highly sensitive technique to study cytogenetic changes. Today, hundreds of commercially available DNA probes serve the basic needs of the biomedical research community. Widespread applications, however, are often limited by the lack of appropriately labeled, specific nucleic acid probes. We describe two approaches for an expeditious preparation of chromosome-specific DNAs and the subsequent probe labeling with reporter molecules of choice. The described techniques allow the preparation of highly specific DNA repeat probes suitable for enumeration of chromosomes in interphase cell nuclei or tissue sections. In addition, there is no need for chromosome enrichment by flow cytometry and sorting or molecular cloning. Our PCR-based method uses either bacterial artificial chromosomes or human genomic DNA as templates with alpha-satellite-specific primers. Here we demonstrate the production of fluorochrome-labeled DNA repeat probes specific for human chromosomes 17 and 18 in just a few days without the need for highly specialized equipment and without the limitation to only a few fluorochrome labels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.