ArticleJournal of bacteriology2006
The highly efficient translation initiation region from the Escherichia coli rpsA gene lacks a shine-dalgarno element.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 47 citations in OpenAlex.
- Stoichiometry-induced differential selection on codon optimization among ribosomal protein genes in bacterial species.Molecular biology and evolution · 2026Article
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- Perturbation of protein homeostasis brings plastids at the crossroad between repair and dismantling.PLoS genetics · 2023Article
- The diversity of Shine-Dalgarno sequences sheds light on the evolution of translation initiation.RNA biology · 2021Review
- Structural basis of sequestration of the anti-Shine-Dalgarno sequence in the Bacteroidetes ribosome.Nucleic acids research · 2021Article
- The Impact of Leadered and Leaderless Gene Structures on Translation Efficiency, Transcript Stability, and Predicted Transcription Rates in Mycobacterium smegmatis.Journal of bacteriology · 2020Article
- Characterization of the internal translation initiation region in monoclonal antibodies expressed inThe Journal of biological chemistry · 2019Article
- Article
- Initiation of mRNA translation in bacteria: structural and dynamic aspects.Cellular and molecular life sciences : CMLS · 2015Review
- Distribution and diversity of ribosome binding sites in prokaryotic genomes.BMC genomics · 2015Article
- Regulation of the rplY gene encoding 5S rRNA binding protein L25 in Escherichia coli and related bacteria.RNA (New York, N.Y.) · 2015Article
- A Novel Quality Measure and Correction Procedure for the Annotation of Microbial Translation Initiation Sites.PloS one · 2015Article
- KLIKK proteases of Tannerella forsythia: putative virulence factors with a unique domain structure.Frontiers in microbiology · 2015Article
- Abundant Intergenic TAACTGA Direct Repeats and Putative Alternate RNA Polymerase β' Subunits in Marine Beggiatoaceae Genomes: Possible Regulatory Roles and Origins.Frontiers in microbiology · 2015Article
- Origin and length distribution of unidirectional prokaryotic overlapping genes.G3 (Bethesda, Md.) · 2014Article
- Robust translation of the nucleoid protein Fis requires a remote upstream AU element and is enhanced by RNA secondary structure.Journal of bacteriology · 2012Article
- S1 ribosomal protein and the interplay between translation and mRNA decay.Nucleic acids research · 2011Article
- The interplay of mRNA stimulatory signals required for AUU-mediated initiation and programmed -1 ribosomal frameshifting in decoding of transposable element IS911.Journal of bacteriology · 2011Article
- Local absence of secondary structure permits translation of mRNAs that lack ribosome-binding sites.PLoS genetics · 2011Article
- Proximity of the start codon to a leaderless mRNA's 5' terminus is a strong positive determinant of ribosome binding and expression in Escherichia coli.Journal of bacteriology · 2010Article
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The translational initiation region (TIR) of the Escherichia coli rpsA gene, which encodes ribosomal protein S1, shows a number of unusual features. It extends far upstream (to position -91) of the initiator AUG, it lacks a canonical Shine-Dalgarno sequence (SD) element, and it can fold into three successive hairpins (I, II, and III) that are essential for high translational activity. Two conserved GGA trinucleotides, present in the loops of hairpins I and II, have been proposed to form a discontinuous SD. Here, we have tested this hypothesis with the "specialized ribosome" approach. Depending upon the constructs used, translation initiation was decreased three- to sevenfold upon changing the conserved GGA to CCU. However, although chemical probing showed that the mutated trinucleotides were accessible, no restoration was observed when the ribosome anti-SD was symmetrically changed from CCUCC to GGAGG. When the same change was introduced in the SD from a conventional TIR as a control, activity was stimulated. This result suggests that the GGA trinucleotides do not form a discontinuous SD. Others hypotheses that may account for their role are discussed. Curiously, we also find that, when expressed at moderate level (30 to 40% of total ribosomes), specialized ribosomes are only twofold disadvantaged over normal ribosomes for the translation of bulk cellular mRNAs. These findings suggest that, under these conditions, the SD-anti-SD interaction plays a significant but not essential role for the synthesis of bulk cellular proteins.
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