ArticleCellular microbiology2006
Gonococci exit apically and basally from polarized epithelial cells and exhibit dynamic changes in type IV pili.
Article in Cellular microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- Development and implementation of a Type I-C CRISPR-based programmable repression system formBio · 2024Article
- Gonococcal invasion into epithelial cells depends on both cell polarity and ezrin.PLoS pathogens · 2021Article
- Folliculin Controls the Intracellular Survival and Trans-Epithelial Passage ofFrontiers in cellular and infection microbiology · 2020Article
- PacBio Amplicon Sequencing Method To Measure Pilin Antigenic Variation Frequencies of Neisseria gonorrhoeae.mSphere · 2019Article
- High-Throughput Particle Uptake Analysis by Imaging Flow Cytometry.Current protocols in cytometry · 2017Article
- Multinucleated Giant Cells Are Specialized for Complement-Mediated Phagocytosis and Large Target Destruction.Cell reports · 2015Article
- An improved method for differentiating cell-bound from internalized particles by imaging flow cytometry.Journal of immunological methods · 2015Article
- Rho GTPases as pathogen targets: Focus on curable sexually transmitted infections.Small GTPases · 2015Review
- Article
- Constitutively Opa-expressing and Opa-deficient neisseria gonorrhoeae strains differentially stimulate and survive exposure to human neutrophils.Journal of bacteriology · 2013Article
- Phase variation leads to the misidentification of a Neisseria gonorrhoeae virulence gene.PloS one · 2013Article
- Pilus phase variation switches gonococcal adherence to invasion by caveolin-1-dependent host cell signaling.PLoS pathogens · 2013Article
- Neisseria gonorrhoeae pilus attenuates cytokine response of human fallopian tube explants.Journal of biomedicine & biotechnology · 2012Article
- Neisseria gonorrhoeae-mediated inhibition of apoptotic signalling in polymorphonuclear leukocytes.Infection and immunity · 2011Article
- The Pathobiology of Neisseria gonorrhoeae Lower Female Genital Tract Infection.Frontiers in microbiology · 2011Article
- Resistance of Neisseria gonorrhoeae to non-oxidative killing by adherent human polymorphonuclear leucocytes.Cellular microbiology · 2009Article
- Neisseria gonorrhoeae suppresses the oxidative burst of human polymorphonuclear leukocytes.Cellular microbiology · 2008Article
- pilQ Missense mutations have diverse effects on PilQ multimer formation, piliation, and pilus function in Neisseria gonorrhoeae.Journal of bacteriology · 2007Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
Type IV pili are a major virulence factor of the obligate human pathogen Neisseria gonorrhoeae (the gonococcus; Gc). Pili facilitate bacterial adherence to epithelial cells, but their participation in later steps of epithelial infection, particularly intracellular replication and exit, is poorly understood. Using polarized T84 cells as a model for mature mucosal epithelia, pilus dynamics in piliated, Opa-expressing Gc were examined over time. T84 infection was characterized by a several-hour delay in the growth of cell-associated bacteria and by non-directional exit of Gc, the first time these phenomena have been reported. During infection, non-piliated progeny arose stochastically from piliated progenitors. Piliated and non-piliated Gc replicated and exited from T84 cell monolayers equally well, demonstrating that piliation did not influence Gc survival during epithelial infection. The frequency with which pilin variants arose from a defined piliated progenitor during T84 cell infection was found to be sufficiently high to account for the extensive pilin variation reported during human infection. However, the repertoire of variants appearing in association with T84 cells was similar to what was seen in the absence of cells, demonstrating that polarized epithelial cells can support Gc replication without selecting for a subset of pilin variants or piliation states.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.