ArticlePlant physiology2006
Computational estimation and experimental verification of off-target silencing during posttranscriptional gene silencing in plants.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
What it found
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Who cites it
91 citing papers in PubMed, 211 citations in OpenAlex.
- MicroRNA-Induced Gene Silencing (MIGS): A Tool for Multi-Gene Silencing and Targeting Viruses in Plants.Plant biotechnology journal · 2026Article
- Silencing of disease susceptibility genes: an effective disease resistance strategy against fungal pathogens.Plant cell reports · 2025Review
- Trans-Kingdom sRNA Silencing inPathogens (Basel, Switzerland) · 2025Review
- RNAi and genome editing of sugarcane: Progress and prospects.The Plant journal : for cell and molecular biology · 2025Review
- Barley stripe mosaic virus-induced gene silencing for functional validation of abiotic stress in barley.Functional & integrative genomics · 2024Review
- RNA Interference Applied to Crustacean Aquaculture.Biomolecules · 2024Review
- Host-Delivered RNA Interference for Durable Pest Resistance in Plants: Advanced Methods, Challenges, and Applications.Molecular biotechnology · 2024Review
- RNAi-Based Therapy: Combating Shrimp Viral Diseases.Viruses · 2023Review
- Virus-Induced Gene Silencing in the Tea Plant (Plants (Basel, Switzerland) · 2023Article
- A Guide to Cannabis Virology: From the Virome Investigation to the Development of Viral Biotechnological Tools.Viruses · 2023Review
- Gold nanoparticles (AuNPs) can rapidly deliver artificial microRNA (AmiRNA)-ATG6 to silence ATG6 expression in Arabidopsis.Plant cell reports · 2023Article
- Virus-Induced Gene Silencing (VIGS): A Powerful Tool for Crop Improvement and Its Advancement towards Epigenetics.International journal of molecular sciences · 2023Review
- Establishing Physalis as a Solanaceae model system enables genetic reevaluation of the inflated calyx syndrome.The Plant cell · 2023Article
- Molecular mechanisms underlying host-induced gene silencing.The Plant cell · 2022Review
- CRISPR-Cas-mediated transcriptional control and epi-mutagenesis.Plant physiology · 2022Review
- Recent Advances in Plant Gene Silencing Methods.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Intragenesis and Synthetic Biology Approach towards Accelerating Genetic Gains on Strawberry: Development of New Tools to Improve Fruit Quality and Resistance to Pathogens.Plants (Basel, Switzerland) · 2021Article
- Polycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber.International journal of molecular sciences · 2021Article
- Inhibition of lateral shoot formation by RNA interference and chemically induced mutations to genes expressed in the axillary meristem of Nicotiana tabacum L.BMC plant biology · 2021Article
- Delivery of miR-26a Using an Exosomes-Based Nanosystem Inhibited Proliferation of Hepatocellular Carcinoma.Frontiers in molecular biosciences · 2021Article
31 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Successful application of posttranscriptional gene silencing (PTGS) for gene function study in both plants and animals depends on high target specificity and silencing efficiency. By computational analysis with genome and/or transcriptome sequences of 25 plant species, we predicted that about 50% to 70% of gene transcripts in plants have potential off-targets when used for PTGS that could obscure experimental results. We have developed a publicly available Web-based computational tool called siRNA Scan to identify potential off-targets during PTGS. Some of the potential off-targets obtained from this tool were tested by measuring the amount of off-target transcripts using quantitative reverse transcription-PCR. Up to 50% of the predicted off-target genes tested in plants were actually silenced when tested experimentally. Our results suggest that a high risk of off-target gene silencing exists during PTGS in plants. Our siRNA Scan tool is useful to design better constructs for PTGS by minimizing off-target gene silencing in both plants and animals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.