ArticleBiophysical journal2006
Nonmuscle myosin IIA-dependent force inhibits cell spreading and drives F-actin flow.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 169 papers.
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Who cites it
169 citing papers in PubMed.
- Mechanical memory of confinement pressure governs expansion size in epithelial monolayers.Life science alliance · 2026Article
- Diverse ancestral myosin motors generate and segregate distinct types of nanocluster-rich domains at the plasma membrane.Science advances · 2026Article
- hsa_circ_0063865 regulates cytoskeleton rearrangement and endoplasmic reticulum stress to promote esophageal tumorigenesis under co-exposure to N-nitrosamines and microcystin.Journal of translational medicine · 2026Article
- Actin-membrane interface stress regulates Arp2/3-branched actin density during lamellipodial protrusion.bioRxiv : the preprint server for biology · 2026Article
- Breaking the link between morphology and potency for mESCs.Cell & bioscience · 2025Article
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- Force transmission is a master regulator of mechanical cell competition.Nature materials · 2025Article
- Force-bearing phagocytic adhesion rings mediate the phagocytosis of surface-bound particles.Nature communications · 2025Article
- Emergence of diverse patterns driven by molecular motors in the motility assay.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Non-Muscle Myosin II A: Friend or Foe in Cancer?International journal of molecular sciences · 2024Review
- Talin2 binds to non-muscle myosin IIa and regulates cell attachment and fibronectin secretion.Scientific reports · 2024Article
- LUZP1 regulates the maturation of contractile actomyosin bundles.Cellular and molecular life sciences : CMLS · 2024Article
- On the role of myosin-induced actin depolymerization during cell migration.Molecular biology of the cell · 2023Article
- Rapid and effective preparation of clonal bone marrow-derived mesenchymal stem/stromal cell sheets to reduce renal fibrosis.Scientific reports · 2023Article
- Non-muscle myosins control radial glial basal endfeet to mediate interneuron organization.PLoS biology · 2023Article
- Ligand functionalization of titanium nanopattern enables the analysis of cell-ligand interactions by super-resolution microscopy.Nature protocols · 2022Review
- Extraction of accurate cytoskeletal actin velocity distributions from noisy measurements.Nature communications · 2022Article
- Analysis of monocyte cell tractions in 2.5D reveals mesoscale mechanics of podosomes during substrate-indenting cell protrusion.Journal of cell science · 2022Article
- Modeling cell protrusion predicts how myosin II and actin turnover affect adhesion-based signaling.Biophysical journal · 2022Article
- Article
109 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Nonmuscle myosin IIA (NMM-IIA) is involved in the formation of focal adhesions and neurite retraction. However, the role of NMM-IIA in these functions remains largely unknown. Using RNA interference as a tool to decrease NMM-IIA expression, we have found that NMM-IIA is the major myosin involved in traction force generation and retrograde F-actin flow in mouse embryonic fibroblast cells. Quantitative analyses revealed that approximately 60% of traction force on fibronectin-coated surfaces is contributed by NMM-IIA and approximately 30% by NMM-IIB. The retrograde F-actin flow decreased dramatically in NMM-IIA-depleted cells, but seemed unaffected by NMM-IIB deletion. In addition, we found that depletion of NMM-IIA caused cells to spread at a higher rate and to a greater area on fibronectin substrates during the early spreading period, whereas deletion of NMM-IIB appeared to have no effect on spreading. The distribution of NMM-IIA was concentrated on the dorsal surface and approached the ventral surface in the periphery, whereas NMM-IIB was primarily concentrated around the nucleus and to a lesser extent at the ventral surface in cell periphery. Our results suggest that NMM-IIA is involved in generating a coherent cytoplasmic contractile force from one side of the cell to the other through the cross-linking and the contraction of dorsal actin filaments.
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