ArticleBiophysical journal2006
Translational diffusion of fluorescent proteins by molecular fourier imaging correlation spectroscopy.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 12 citations in OpenAlex.
- Patterns of intersecting fiber arrays revealed in whole muscle with generalized Q-space imaging.Biophysical journal · 2015Article
- Single-molecule FRET and linear dichroism studies of DNA breathing and helicase binding at replication fork junctions.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Subcellular dynamics and protein conformation fluctuations measured by Fourier imaging correlation spectroscopy.Annual review of physical chemistry · 2010Review
- I. Conformational dynamics of biological macromolecules by polarization-modulated Fourier imaging correlation spectroscopy.The journal of physical chemistry. B · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The ability to noninvasively observe translational diffusion of proteins and protein complexes is important to many biophysical problems. We report high signal/noise (>or=250) measurements of the translational diffusion in viscous solution of the fluorescent protein, DsRed. This is carried out using a new technique: molecular Fourier imaging correlation spectroscopy (M-FICS). M-FICS is an interferometric method that detects a collective Fourier component of the fluctuating density of a small population of fluorescent molecules, and provides information about the distribution of molecular diffusivities. A theoretical analysis is presented that expresses the detected signal fluctuations in terms of the relevant time-correlation functions for molecular translational diffusion. Furthermore, the role played by optical orientational degrees of freedom is established. We report Fickian self-diffusion of the DsRed tetramer at short timescales. The long-time deviation of our data from Fickian behavior is used to determine the variance of the distribution of the protein self-diffusion coefficient. We compare our results to the expected outcomes for 1), a bi-disperse distribution of protein species, and 2), dynamic disorder of the host solvent.
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Registered trials
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