ArticleBiotechnology and bioengineering2007
Microparticle adhesive dynamics and rolling mediated by selectin-specific antibodies under flow.
Article in Biotechnology and bioengineering, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Adhesion patterns in the microvasculature are dependent on bifurcation angle.Microvascular research · 2015Article
- Article
- Expression of E-selectin ligands on circulating tumor cells: cross-regulation with cancer stem cell regulatory pathways?Frontiers in oncology · 2012Article
- Using engineered single-chain antibodies to correlate molecular binding properties and nanoparticle adhesion dynamics.Langmuir : the ACS journal of surfaces and colloids · 2011Article
- Bifurcations: focal points of particle adhesion in microvascular networks.Microcirculation (New York, N.Y. : 1994) · 2011Article
- Flow and adhesion of drug carriers in blood vessels depend on their shape: a study using model synthetic microvascular networks.Journal of controlled release : official journal of the Controlled Release Society · 2010Article
- The Effects of Load on E-Selectin Bond Rupture and Bond Formation.Cellular and molecular bioengineering · 2010Article
- Action at a distance: lengthening adhesion bonds with poly(ethylene glycol) spacers enhances mechanically stressed affinity for improved vascular targeting of microparticles.Langmuir : the ACS journal of surfaces and colloids · 2009Article
- Imaging E-selectin expression following traumatic brain injury in the rat using a targeted USPIO contrast agent.Magma (New York, N.Y.) · 2009Article
- Flow dynamics, binding and detachment of spherical carriers targeted to ICAM-1 on endothelial cells.Biorheology · 2009Article
- Functional binding of human adipose-derived stromal cells: effects of extraction method and hypoxia pretreatment.Annals of plastic surgery · 2008Article
- Enhancement of L-selectin, but not P-selectin, bond formation frequency by convective flow.Biophysical journal · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
In vitro studies were performed to characterize the relative performance of candidate receptors to target microparticles to inflammatory markers on vascular endothelium. To model the interactions of drug-bearing microparticles or imaging contrast agents with the vasculature, 6 micron polystyrene particles bearing antibodies, peptides, or carbohydrates were perfused over immobilized E- or P-selectin in a flow chamber. Microparticles conjugated with HuEP5C7.g2 (HuEP), a monoclonal antibody (mAb) specific to E- and P-selectin, supported leukocyte-like rolling and transient adhesion at venular shear rates. In contrast, microparticles conjugated with a higher affinity mAb specific for P-selectin (G1) were unable to form bonds at venular flow rates. When both HuEP and G1 were conjugated to the microparticle, HuEP supported binding to P-selectin in flow which allowed G1 to form bonds leading to stable adhesion. While the microparticle attachment and rolling performance was not as stable as that mediated by the natural ligands P-selectin Glycoprotein Ligand-1 or sialyl Lewis(x), HuEP performed significantly better than any previously characterized mAb in terms of mediating microparticle binding under flow conditions. HuEP may be a viable alternative to natural ligands to selectins for targeting particles to inflamed endothelium.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.