ArticleCurrent genetics2006
Expression of heterologous aquaporins for functional analysis in Saccharomyces cerevisiae.
Article in Current genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- A Semi-Quantitative Yeast Complementation Platform for Characterizing Urea and Ammonia Transport by Membrane Channels.Current protocols · 2026Article
- Major Intrinsic Proteins in Fungi: A Special Emphasis on the XIP Subfamily.Journal of fungi (Basel, Switzerland) · 2025Review
- Yeast Complementation Assays Demonstrating the Importance of the Affinity Tag Position in Membrane Protein Purification, as Exemplified bySmall science · 2025Article
- The Arabidopsis PM19L1 Protein Functions as a Regulator of Germination Under Osmotic Stress.Plant direct · 2025Article
- Characterization of human aquaporin ion channels in a yeast expression system as a tool for novel ion channel discovery.Bioscience reports · 2024Article
- Yeast as a tool for membrane protein production and structure determination.FEMS yeast research · 2022Article
- Ectopic Expression ofInternational journal of molecular sciences · 2021Article
- Functional Analysis of Aquaporin Water Permeability Using anFrontiers in bioengineering and biotechnology · 2020Article
- Prokaryotic Aquaporins.Cells · 2019Review
- Aquaporin-graphene interface: relevance to point-of-care device for renal cell carcinoma and desalination.Interface focus · 2018Review
- Quantification of the Intracellular Life Time of Water Molecules to Measure Transport Rates of Human Aquaglyceroporins.The Journal of membrane biology · 2017Article
- Rat Aquaporin-5 Is pH-Gated Induced by Phosphorylation and Is Implicated in Oxidative Stress.International journal of molecular sciences · 2016Article
- Detecting Aquaporin Function and Regulation.Frontiers in chemistry · 2016Review
- Aquaporins are critical for provision of water during lactation and intrauterine progeny hydration to maintain tsetse fly reproductive success.PLoS neglected tropical diseases · 2014Article
- Article
- Saccharomyces cerivisiae as a model system for kidney disease: what can yeast tell us about renal function?American journal of physiology. Renal physiology · 2011Article
- Article
- A yeast-based phenotypic screen for aquaporin inhibitors.Pflugers Archiv : European journal of physiology · 2008Article
- A subgroup of plant aquaporins facilitate the bi-directional diffusion of As(OH)3 and Sb(OH)3 across membranes.BMC biology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study the yeast Saccharomyces cerevisiae, which is a genetically tractable model for analysis of osmoregulation, has been used for analysis of heterologous aquaporins. Aquaporin water channels play important roles in the control of water homeostasis in individual cells and multicellular organisms. We have investigated the effects of functional expression of the mammalian aquaporins AQP1 and AQP5 and the aquaglyceroporins AQP3 and AQP9. Expression of aquaporins caused moderate growth inhibition under hyperosmotic stress, while expression of aquaglyceroporins mediated strong growth inhibition due to glycerol loss. Water transport was monitored in protoplasts, where the kinetics of bursting was influenced by presence of aquaporins but not aquaglyceroporins. We observed glycerol transport through aquaglyceroporins, but not aquaporins, in a yeast strain deficient in glycerol production, whose growth depends on glycerol inflow. In addition, a gene reporter assay allowed to indirectly monitor the effect of AQP9-mediated enhanced glycerol loss on osmoadaptation. Transport activity of certain aqua(glycero)porins was diminished by low pH or CuSO4, suggesting that yeast can potentially be used for screening of putative aquaporin inhibitors. We conclude that yeast is a versatile system for functional studies of aquaporins, and it can be developed to screen for compounds of potential pharmacological use.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.