ArticleMolecular and cellular biology2006
Complex minisatellite rearrangements generated in the total or partial absence of Rad27/hFEN1 activity occur in a single generation and are Rad51 and Rad52 dependent.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- RPA and Pif1 cooperate to remove G-rich structures at both leading and lagging strand.Cell stress · 2020Article
- Whole genomic DNA sequencing and comparative genomic analysis of Arthrospira platensis: high genome plasticity and genetic diversity.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
- Intrastrand triplex DNA repeats in bacteria: a source of genomic instability.Nucleic acids research · 2015Article
- The FEN1 E359K germline mutation disrupts the FEN1-WRN interaction and FEN1 GEN activity, causing aneuploidy-associated cancers.Oncogene · 2015Article
- Mutational landscape of yeast mutator strains.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Alleles of the homologous recombination gene, RAD59, identify multiple responses to disrupted DNA replication in Saccharomyces cerevisiae.BMC microbiology · 2013Article
- Novel checkpoint pathway organization promotes genome stability in stationary-phase yeast cells.Molecular and cellular biology · 2013Article
- Functional analyses of human DNA repair proteins important for aging and genomic stability using yeast genetics.DNA repair · 2012Review
- Stimulation of gross chromosomal rearrangements by the human CEB1 and CEB25 minisatellites in Saccharomyces cerevisiae depends on G-quadruplexes or Cdc13.PLoS genetics · 2012Article
- G-quadruplex-induced instability during leading-strand replication.The EMBO journal · 2011Article
- Functional regulation of FEN1 nuclease and its link to cancer.Nucleic acids research · 2011Review
- Genetic and functional interactions between Mus81-Mms4 and Rad27.Nucleic acids research · 2010Article
- Genetic instability triggered by G-quadruplex interacting Phen-DC compounds in Saccharomyces cerevisiae.Nucleic acids research · 2010Article
- The yeast Pif1 helicase prevents genomic instability caused by G-quadruplex-forming CEB1 sequences in vivo.PLoS genetics · 2009Article
- Comparative genomics and molecular dynamics of DNA repeats in eukaryotes.Microbiology and molecular biology reviews : MMBR · 2008Review
- Megasatellites: a peculiar class of giant minisatellites in genes involved in cell adhesion and pathogenicity in Candida glabrata.Nucleic acids research · 2008Article
- Sequence-based estimation of minisatellite and microsatellite repeat variability.Genome research · 2007Article
- Synthesis-dependent strand annealing in meiosis.PLoS biology · 2007Article
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Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genomes contain tandem repeat blocks that are at risk of expansion or contraction. The mechanisms of destabilization of the human minisatellite CEB1 (arrays of 36- to 43-bp repeats) were investigated in a previously developed model system, in which CEB1-0.6 (14 repeats) and CEB1-1.8 (42 repeats) alleles were inserted into the genome of Saccharomyces cerevisiae. As in human cells, CEB1 is stable in mitotically growing yeast cells but is frequently rearranged in the absence of the Rad27/hFEN1 protein involved in Okazaki fragments maturation. To gain insight into this mode of destabilization, the CEB1-1.8 and CEB1-0.6 human alleles and 47 rearrangements derived from a CEB1-1.8 progenitor in rad27Delta cells were sequenced. A high degree of polymorphism of CEB1 internal repeats was observed, attesting to a large variety of homology-driven rearrangements. Simple deletion, double deletion, and highly complex events were observed. Pedigree analysis showed that all rearrangements, even the most complex, occurred in a single generation and were inherited equally by mother and daughter cells. Finally, the rearrangement frequency was found to increase with array size, and partial complementation of the rad27Delta mutation by hFEN1 demonstrated that the production of novel CEB1 alleles is Rad52 and Rad51 dependent. Instability can be explained by an accumulation of unresolved flap structures during replication, leading to the formation of recombinogenic lesions and faulty repair, best understood by homology-dependent synthesis-strand displacement and annealing.
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