ArticleJournal of virology2006
Human cytomegalovirus pUS24 is a virion protein that functions very early in the replication cycle.
Article in Journal of virology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Review
- Inferring differential subcellular localisation in comparative spatial proteomics using BANDLE.Nature communications · 2022Article
- Human Cytomegalovirus Primary Infection and Reactivation: Insights From Virion-Carried Molecules.Frontiers in microbiology · 2020Review
- Synthetic lethal mutations in the cyclin A interface of human cytomegalovirus.PLoS pathogens · 2017Article
- THY-1 Cell Surface Antigen (CD90) Has an Important Role in the Initial Stage of Human Cytomegalovirus Infection.PLoS pathogens · 2015Article
- The life cycle and pathogenesis of human cytomegalovirus infection: lessons from proteomics.Expert review of proteomics · 2014Article
- Article
- Deletion of the human cytomegalovirus US17 gene increases the ratio of genomes per infectious unit and alters regulation of immune and endoplasmic reticulum stress response genes at early and late times after infection.Journal of virology · 2014Article
- Functional annotation of human cytomegalovirus gene products: an update.Frontiers in microbiology · 2014Review
- The US16 gene of human cytomegalovirus is required for efficient viral infection of endothelial and epithelial cells.Journal of virology · 2012Article
- Cytomegalovirus UL103 controls virion and dense body egress.Journal of virology · 2011Article
- Categorical data analysis in experimental biology.Developmental biology · 2010Review
- Article
- Cyclin-dependent kinase activity controls the onset of the HCMV lytic cycle.PLoS pathogens · 2010Article
- Human cytomegalovirus UL29/28 protein interacts with components of the NuRD complex which promote accumulation of immediate-early RNA.PLoS pathogens · 2010Article
- Human cytomegalovirus UL28 and UL29 open reading frames encode a spliced mRNA and stimulate accumulation of immediate-early RNAs.Journal of virology · 2009Article
- Inhibition of cyclooxygenase activity blocks cell-to-cell spread of human cytomegalovirus.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Dynamic histone H3 acetylation and methylation at human cytomegalovirus promoters during replication in fibroblasts.Journal of virology · 2008Article
- Establishment of a cell-based assay for screening of compounds inhibiting very early events in the cytomegalovirus replication cycle and characterization of a compound identified using the assay.Antimicrobial agents and chemotherapy · 2008Article
- Tegument proteins of human cytomegalovirus.Microbiology and molecular biology reviews : MMBR · 2008Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have characterized the function of the human cytomegalovirus US24 gene, a US22 gene family member. Two US24-deficient mutants (BADinUS24 and BADsubUS24) exhibited a 20- to 30-fold growth defect, compared to their wild-type parent (BADwt), after infection at a relatively low (0.01 PFU/cell) or high (1 PFU/cell) input multiplicity. Representative virus-encoded proteins and viral DNA accumulated with normal kinetics to wild-type levels after infection with mutant virus when cells received equal numbers of mutant and wild-type infectious units. Further, the proteins were properly localized and no ultrastructural differences were found by electron microscopy in mutant-virus-infected cells compared to wild-type-virus-infected cells. However, virions produced by US24-deficient mutants had a 10-fold-higher genome-to-PFU ratio than wild-type virus. When infections were performed using equal numbers of input virus particles, the expression of immediate-early, early, and late viral proteins was substantially delayed and decreased in the absence of US24 protein. This delay is not due to inefficient virus entry, since two tegument proteins and viral DNA moved to the nucleus equally well in mutant- and wild-type-virus-infected cells. In summary, US24 is a virion protein and virions produced by US24-deficient viruses exhibit a block to the human cytomegalovirus replication cycle after viral DNA reaches the nucleus and before immediate-early mRNAs are transcribed.
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