ArticleProceedings of the National Academy of Sciences of the United States of America2006
Bestrophin-2 is a candidate calcium-activated chloride channel involved in olfactory transduction.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 115 citations in OpenAlex.
- Pleiotropic Effects of the NSAID Fenamates on Chloride Channels: Opportunity for Ion Channelopathies?Pharmacology research & perspectives · 2025Review
- Structure and Function of the Bestrophin family of calcium-activated chloride channels.Channels (Austin, Tex.) · 2021Review
- Functional expression of TMEM16A in taste bud cells.The Journal of physiology · 2021Article
- The cyclic AMP signaling pathway in the rodent main olfactory system.Cell and tissue research · 2021Review
- Exploring the molecular basis of neuronal excitability in a vocal learner.BMC genomics · 2019Article
- TMEM16A calcium-activated chloride currents in supporting cells of the mouse olfactory epithelium.The Journal of general physiology · 2019Article
- The long tale of the calcium activated ClChannels (Austin, Tex.) · 2017Review
- Distribution and Function of the Bestrophin-1 (Best1) Channel in the Brain.Experimental neurobiology · 2017Review
- A bestrophin-like protein modulates the proton motive force across the thylakoid membrane in Arabidopsis.Journal of integrative plant biology · 2016Article
- Single Ca(2+)-activated Cl(-) channel currents recorded from toad olfactory cilia.BMC neuroscience · 2016Article
- Molecular and functional significance of Ca(2+)-activated Cl(-) channels in pulmonary arterial smooth muscle.Pulmonary circulation · 2015Review
- Conditional knockout of TMEM16A/anoctamin1 abolishes the calcium-activated chloride current in mouse vomeronasal sensory neurons.The Journal of general physiology · 2015Article
- RNA-Seq Analysis of Human Trigeminal and Dorsal Root Ganglia with a Focus on Chemoreceptors.PloS one · 2015Article
- Article
- Niflumic acid affects store-operated Ca(2+)-permeable (SOC) and Ca (2+)-dependent K (+) and Cl (-) ion channels and induces apoptosis in K562 cells.The Journal of membrane biology · 2014Article
- Interactions between permeation and gating in the TMEM16B/anoctamin2 calcium-activated chloride channel.The Journal of general physiology · 2014Article
- The bestrophin- and TMEM16A-associated Ca(2+)- activated Cl(–) channels in vascular smooth muscles.Channels (Austin, Tex.) · 2014Review
- NanoCAGE analysis of the mouse olfactory epithelium identifies the expression of vomeronasal receptors and of proximal LINE elements.Frontiers in cellular neuroscience · 2014Article
- Channel properties of the splicing isoforms of the olfactory calcium-activated chloride channel Anoctamin 2.The Journal of general physiology · 2013Article
- Channel-mediated astrocytic glutamate release via Bestrophin-1 targets synaptic NMDARs.Molecular brain · 2013Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ca-activated Cl channels are an important component of olfactory transduction. Odor binding to olfactory receptors in the cilia of olfactory sensory neurons (OSNs) leads to an increase of intraciliary Ca concentration by Ca entry through cyclic nucleotide-gated (CNG) channels. Ca activates a Cl channel that leads to an efflux of Cl from the cilia, contributing to the amplification of the OSN depolarization. The molecular identity of this Cl channel remains elusive. Recent evidence has indicated that bestrophins are able to form Ca-activated Cl channels in heterologous systems. Here we have analyzed the expression of bestrophins in the mouse olfactory epithelium and demonstrated that only mouse bestrophin-2 (mBest2) was expressed. Single-cell RT-PCR showed that mBest2 was expressed in OSNs but not in supporting cells. Immunohistochemistry revealed that mBest2 was expressed on the cilia of OSNs, the site of olfactory transduction, and colocalized with the main CNGA2 channel subunit. Electrophysiological properties of Ca-activated Cl currents from native channels in dendritic knob/cilia of mouse OSNs were compared with those induced by the expression of mBest2 in HEK-293 cells. We found the same anion permeability sequence, small estimated single-channel conductances, a Ca sensitivity difference of one order of magnitude, and the same side-specific blockage of the two Cl channel blockers commonly used to inhibit the odorant-induced Ca-activated Cl current in OSNs, niflumic acid, and 4-acetamido-4'-isothiocyanato-stilben-2,2'-disulfonate (SITS). Therefore, our data suggest that mBest2 is a good candidate for being a molecular component of the olfactory Ca-activated Cl channel.
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