ArticleThe Journal of cell biology1990
Aberrant regulation of MyoD1 contributes to the partially defective myogenic phenotype of BC3H1 cells.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 106 citations in OpenAlex.
- Survival motor neuron protein deficiency impairs myotube formation by altering myogenic gene expression and focal adhesion dynamics.Human molecular genetics · 2014Article
- miR-186 inhibits muscle cell differentiation through myogenin regulation.The Journal of biological chemistry · 2014Article
- The role of pannexin hemichannels in inflammation and regeneration.Frontiers in physiology · 2014Review
- Effects of myogenin on expression of late muscle genes through MyoD-dependent chromatin remodeling ability of myogenin.Molecules and cells · 2012Article
- Chemokine-like receptor 1 regulates skeletal muscle cell myogenesis.American journal of physiology. Cell physiology · 2012Article
- Barx2 is expressed in satellite cells and is required for normal muscle growth and regeneration.Stem cells (Dayton, Ohio) · 2012Article
- MyoD directly up-regulates premyogenic mesoderm factors during induction of skeletal myogenesis in stem cells.The Journal of biological chemistry · 2011Article
- Myogenic factors that regulate expression of muscle-specific microRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Wnt10b deficiency promotes coexpression of myogenic and adipogenic programs in myoblasts.Molecular biology of the cell · 2005Article
- Accelerated response of the myogenin gene to denervation in mutant mice lacking phosphorylation of myogenin at threonine 87.Molecular and cellular biology · 2004Article
- Aberrant development of motor axons and neuromuscular synapses in MyoD-null mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- MyoD distal regulatory region contains an SRF binding CArG element required for MyoD expression in skeletal myoblasts and during muscle regeneration.Molecular biology of the cell · 2003Article
- Myostatin levels in regenerating rat muscles and in myogenic cell cultures.Journal of muscle research and cell motility · 2000Article
- Reduced differentiation potential of primary MyoD-/- myogenic cells derived from adult skeletal muscle.The Journal of cell biology · 1999Article
- RhoA GTPase and serum response factor control selectively the expression of MyoD without affecting Myf5 in mouse myoblasts.Molecular biology of the cell · 1998Article
- Role of insulin-like growth factors and myogenin in the altered program of proliferation and differentiation in the NFB4 mutant muscle cell line.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Myosin light chain 3F regulatory sequences confer regionalized cardiac and skeletal muscle expression in transgenic mice.The Journal of cell biology · 1995Article
- Somite subdomains, muscle cell origins, and the four muscle regulatory factor proteins.The Journal of cell biology · 1994Article
- Regulation of the GLUT1 glucose transporter in cultured myocytes: total number and subcellular distribution as determined by photoaffinity labelling.The Biochemical journal · 1994Article
- Inhibition of desmin expression blocks myoblast fusion and interferes with the myogenic regulators MyoD and myogenin.The Journal of cell biology · 1994Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Two skeletal muscle-specific regulatory factors, myogenin and MyoD1, share extensive homology within a myc similarity region and have each been shown to activate the morphologic and molecular events associated with myogenesis after transfection into nonmyogenic cells. The BC3H1 muscle cell line expresses myogenin and other muscle-specific genes, but does not express MyoD1 during differentiation. BC3H1 cells also do not upregulate alpha-cardiac actin or fast myosin light chain, nor do they form multinucleate myotubes during differentiation. In this study, we examined the basis for the lack of MyoD1 expression in BC3H1 cells and investigated whether their failure to express MyoD1 is responsible for their defects in differentiation. We report that expression of an exogenous MyoD1 cDNA in BC3H1 cells was sufficient to elevate the expression of alpha-cardiac actin and fast myosin light chain, and to convert these cells to a phenotype that forms multinucleate myotubes during differentiation. Whereas myogenin and MyoD1 positively regulated their own expression in transfected 10T1/2 cells, they could not, either alone or in combination, activate MyoD1 expression in BC3H1 cells. Exposure of BC3H1 cells to 5-azacytidine also failed to activate MyoD1 expression or to rescue the cell's ability to fuse. These results suggest that BC3H1 cells may possess a defect that prevents activation of the MyoD1 gene by MyoD1 or myogenin. That an exogenous MyoD1 gene could rescue those aspects of the differentiation program that are defective in BC3H1 cells also suggests that the actions of MyoD1 and myogenin are not entirely redundant and that MyoD1 may be required for activation of the complete repertoire of events associated with myogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.