ArticleThe Journal of membrane biology2006
Strengths and limits of Beta distributions as a means of reconstructing the true single-channel current in patch clamp time series with fast gating.
Article in The Journal of membrane biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 20 citations in OpenAlex.
- Similarity of the ion occupations of KChannels (Austin, Tex.) · 2026Article
- Binding kinetics of quaternary ammonium ions in Kcv potassium channels.Channels (Austin, Tex.) · 2024Article
- Distinct lipid bilayer compositions have general and protein-specific effects on K+ channel function.The Journal of general physiology · 2021Article
- Asymmetric Interplay Between KFrontiers in physiology · 2021Article
- High bandwidth approaches in nanopore and ion channel recordings - A tutorial review.Analytica chimica acta · 2019Review
- Extended beta distributions open the access to fast gating in bilayer experiments-assigning the voltage-dependent gating to the selectivity filter.FEBS letters · 2017Article
- How to resolve microsecond current fluctuations in single ion channels: the power of beta distributions.Channels (Austin, Tex.) · 2015Review
- Ca2+ block and flickering both contribute to the negative slope of the IV curve in BK channels.The Journal of general physiology · 2013Article
- Lack of negative slope in I-V plots for BK channels at positive potentials in the absence of intracellular blockers.The Journal of general physiology · 2013Article
- Modulation of enrofloxacin binding in OmpF by Mg2+ as revealed by the analysis of fast flickering single-porin current.The Journal of general physiology · 2012Article
- Using a five-state model for fitting amplitude histograms from MaxiK channels: beta-distributions reveal more than expected.European biophysics journal : EBJ · 2009Article
- Fast and slow gating are inherent properties of the pore module of the K+ channel Kcv.The Journal of general physiology · 2009Article
- Interference of shot noise of open-channel current with analysis of fast gating: patchers do not (Yet) have to care.The Journal of membrane biology · 2009Article
- Four-mode gating model of fast inactivation of sodium channel Nav1.2a.Pflugers Archiv : European journal of physiology · 2008Article
- Tl+-induced micros gating of current indicates instability of the MaxiK selectivity filter as caused by ion/pore interaction.The Journal of general physiology · 2008Article
- Saturation and microsecond gating of current indicate depletion-induced instability of the MaxiK selectivity filter.The Journal of general physiology · 2007Article
- The power of two-dimensional dwell-time analysis for model discrimination, temporal resolution, multichannel analysis and level detection.The Journal of membrane biology · 2006Article
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Authors and funding
2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Single-channel current seems to be one of the most obvious characteristics of ion transport. But in some cases, its determination is more complex than anticipated at first glance. Problems arise from fast gating in time series of patch-clamp current, which can lead to a reduced apparent (measured) single-channel current. Reduction is caused by undetected averaging over closed and open intervals in the anti-aliasing filter. Here it is shown that fitting the measured amplitude histograms by Beta distributions is an efficient tool of reconstructing the true current level from measured data. This approach becomes even more powerful when it is applied to amplitude distributions-per-level. Simulated time series are employed to show that the error sum is a good guideline for finding the correct current level. Furthermore, they show that a Markov model smaller than the one used for gating analysis can be used for current determination (mostly O-C, i.e., open-closed). This increases the reliability of the Beta fit. The knowledge of the true current level is not only important for the understanding of the biophysical properties of the channel. It is also a prerequisite for the correct determination of the rate constants of gating. The approach is applied to measured data. The examples reveal the limits of the analysis imposed by the signal-to-noise ratio and the shape of the amplitude distribution. One application shows that the negative slope of the I-V curve of the human MaxiK channel expressed in HEK293 cells is caused by fast gating.
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