ArticleChromosoma2006
The ultrastructure of the kinetochore and kinetochore fiber in Drosophila somatic cells.
Article in Chromosoma, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 66 citations in OpenAlex.
- <italic>Zea mays</italic> Meiotic Spindle Ultrastructure Reveals Kinetochore-Microtubule Interface and Embedded Membrane Components.Cytogenetic and genome research · 2025Article
- An evolutionary perspective on the relationship between kinetochore size and CENP-E dependence for chromosome alignment.Journal of cell science · 2024Article
- Alignment of a Trivalent Chromosome on the Metaphase Plate Is Associated with Differences in Microtubule Density at Each Kinetochore.International journal of molecular sciences · 2024Article
- A farnesyl-dependent structural role for CENP-E in expansion of the fibrous corona.The Journal of cell biology · 2024Article
- Centromere structure and function: lessons from Drosophila.Genetics · 2023Article
- Review
- The Role of Mitotic Kinases and the RZZ Complex in Kinetochore-Microtubule Attachments: Doing the Right Link.Frontiers in cell and developmental biology · 2022Review
- TEM Imaging of Membrane Choreography During Mitosis of Drosophila Tissue Culture Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Increased Expression of Maturation Promoting Factor Components Speeds Up Meiosis in Oocytes from Aged Females.International journal of molecular sciences · 2018Article
- Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors.BMC biology · 2018Article
- Chromosome Segregation Is Biased by Kinetochore Size.Current biology : CB · 2018Article
- Kinesin-5 mediated chromosome congression in insect spindles.Cellular and molecular bioengineering · 2018Article
- Measuring mitotic forces.Methods in cell biology · 2018Article
- The Drosophila orthologue of the INT6 onco-protein regulates mitotic microtubule growth and kinetochore structure.PLoS genetics · 2017Article
- Chromosome biorientation produces hundreds of piconewtons at a metazoan kinetochore.Nature communications · 2016Article
- Article
- Aurora B spatially regulates EB3 phosphorylation to coordinate daughter cell adhesion with cytokinesis.The Journal of cell biology · 2013Article
- Conserved and divergent features of kinetochores and spindle microtubule ends from five species.The Journal of cell biology · 2013Article
- Functional analysis of centrosomal kinase substrates in Drosophila melanogaster reveals a new function of the nuclear envelope component otefin in cell cycle progression.Molecular and cellular biology · 2012Article
- The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells.Molecular biology of the cell · 2012Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Drosophila melanogaster is a widely used model organism for the molecular dissection of mitosis in animals. However, despite the popularity of this system, no studies have been published on the ultrastructure of Drosophila kinetochores and kinetochore fibers (K-fibers) in somatic cells. To amend this situation, we used correlative light (LM) and electron microscopy (EM) to study kinetochores in cultured Drosophila S2 cells during metaphase, and after colchicine treatment to depolymerize all microtubules (MTs). We find that the structure of attached kinetochores in S2 cells is indistinct, consisting of an amorphous inner zone associated with a more electron-dense peripheral surface layer that is approximately 40-50 nm thick. On average, each S2 kinetochore binds 11+/-2 MTs, in contrast to the 4-6 MTs per kinetochore reported for Drosophila spermatocytes. Importantly, nearly all of the kinetochore MT plus ends terminate in the peripheral surface layer, which we argue is analogous to the outer plate in vertebrate kinetochores. Our structural observations provide important data for assessing the results of RNAi studies of mitosis, as well as for the development of mathematical modelling and computer simulation studies in Drosophila and related organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.