ArticleMarine biotechnology (New York, N.Y.)
A cell line (HEW) from embryos of haddock (Melanogrammus aeglefinius) and its capacity to tolerate environmental extremes.
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Rapid genome modifications including chromosomal fusions and large-scale inversions are key features in Arctic codfish species.Genome biology · 2026Article
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- Differentiation and Maturation of Muscle and Fat Cells in Cultivated Seafood: Lessons from Developmental Biology.Marine biotechnology (New York, N.Y.) · 2023Review
- Establishment of a new fish cell line from the brain of humpback grouper (Cromileptes altivelis) and its application in toxicology and bacterial susceptibility.Fish physiology and biochemistry · 2021Article
- Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla.Cell biology international · 2020Article
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- Development and Characterization of a New Cell Line from Olive FlounderDevelopment & reproduction · 2018Article
- Responses of rainbow trout intestinal epithelial cells to different kinds of nutritional deprivation.Fish physiology and biochemistry · 2018Article
- Establishment and characterization of a heart-derived cell line from goldfish (Carassius auratus).Fish physiology and biochemistry · 2017Article
- Establishment from the snout and kidney of goldfish, Carassius auratus, of two new cell lines and their susceptibility to infectious pancreatic necrosis virus.Fish physiology and biochemistry · 2016Article
- Senescence-associated β-galactosidase staining in fish cell lines and primary cultures from several tissues and species, including rainbow trout coelomic fluid and milt.In vitro cellular & developmental biology. Animal · 2015Article
- Cytotoxicity evaluation of silica nanoparticles using fish cell lines.In vitro cellular & developmental biology. Animal · 2014Article
- Derivation and osmotolerance characterization of three immortalized tilapia (Oreochromis mossambicus) cell lines.PloS one · 2014Article
- Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara).Fish physiology and biochemistry · 2012Article
- Development, characterization, conservation and storage of fish cell lines: a review.Fish physiology and biochemistry · 2011Review
- Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation.In vitro cellular & developmental biology. Animal · 2011Article
- Development and characterization of two new cell lines from milkfish (Chanos chanos) and grouper (Epinephelus coioides) for virus isolation.Marine biotechnology (New York, N.Y.)Article
- Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara).Marine biotechnology (New York, N.Y.)Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell lines can be useful experimental tools for studying marine fish, which are often difficult to routinely obtain and maintain in the laboratory. As few cell lines are available from coldwater marine fish, cultures were initiated from late gastrula embryos of haddock (Melanogrammus aeglefinus) in Leibovitz's L-15 with fetal bovine serum (FBS). From one culture, a cell line (HEW) emerged that has been grown for close to 100 population doublings, was heteroploid, and expressed telomerase activity, all of which suggest HEW is immortal. Growth occurred only if FBS was present and was optimal at 12 to 18 degrees C. Usually most cells had an epithelial-like morphology, but under some conditions, cells drew up into round central bodies from which radiated cytoplasmic extensions with multiple branches. These neural-like cells appeared within a few hours of cultures being placed at 28 degrees C or being switch to a simple salt solution (SSS). At 28 degrees C, cells died within 24 h. In SSS, HEW cells survived as a monolayer for at least 7 days. The sensitivity of HEW cells to morphological change and their capacity to withstand starvation should make them useful for investigating cellular responses to environmental stresses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.