ArticleMolecular and cellular biology2006
Sir3 C-terminal domain involvement in the initiation and spreading of heterochromatin.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- The mre11 A470 alleles influence the hereditability and the segregation of telosomes in Saccharomyces cerevisiae.PloS one · 2017Article
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- Hypothesis: Paralog Formation from Progenitor Proteins and Paralog Mutagenesis Spur the Rapid Evolution of Telomere Binding Proteins.Frontiers in genetics · 2016Article
- Combined Interactions of Plant Homeodomain and Chromodomain Regulate NuA4 Activity at DNA Double-Strand Breaks.Genetics · 2016Article
- Heat stress-induced Cup9-dependent transcriptional regulation of SIR2.Molecular and cellular biology · 2015Article
- Dimerization of Sir3 via its C-terminal winged helix domain is essential for yeast heterochromatin formation.The EMBO journal · 2013Article
- Evolutionary analysis of heterochromatin protein compatibility by interspecies complementation in Saccharomyces.Genetics · 2012Article
- Structure and function in the budding yeast nucleus.Genetics · 2012Review
- Structural basis of silencing: Sir3 BAH domain in complex with a nucleosome at 3.0 Å resolution.Science (New York, N.Y.) · 2011Article
- Structural basis for the role of the Sir3 AAA+ domain in silencing: interaction with Sir4 and unmethylated histone H3K79.Genes & development · 2011Article
- Clustering heterochromatin: Sir3 promotes telomere clustering independently of silencing in yeast.The Journal of cell biology · 2011Article
- Chromatin higher-order structure and dynamics.Cold Spring Harbor perspectives in biology · 2010Review
- Silent information regulator 3: the Goldilocks of the silencing complex.Genes & development · 2010Review
- Inactivation of the Sas2 histone acetyltransferase delays senescence driven by telomere dysfunction.The EMBO journal · 2010Article
- Silent chromatin at the middle and ends: lessons from yeasts.The EMBO journal · 2009Review
- Sir3-nucleosome interactions in spreading of silent chromatin in Saccharomyces cerevisiae.Molecular and cellular biology · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Heterochromatin is nucleated at a specific site and subsequently spreads into distal sequences through multiple interactions between modified histones and nonhistone proteins. In the yeast Saccharomyces cerevisiae, these nonhistone proteins include Sir2, Sir3, and Sir4. We have previously shown that loss of the C-terminal Rap1 domain containing Sir3 and Sir4 association sites can be overcome by tethering a 144-amino-acid C-terminal domain (CTD) of Sir3 adjacent to the telomere. Here, we explore the substructure and functions of the CTD. We demonstrate that the CTD is the minimum domain for Sir3 homodimerization, a function that is conserved in related yeasts. However, CTD heterodimers associate at only low efficiencies and correspondingly have low levels of tethered silencing, consistent with an essential role for dimerization in tethered silencing. Six missense alleles were generated, with ctd-Y964A producing the most extreme phenotypes when tethered to the LexA binding sites. Although ctd-Y964A is capable of dimerization, telomere silencing is abrogated, indicating that the CTD serves a second essential function in silencing. Chromatin immunoprecipitation analyses of wild-type and ctd-Y964A mutant cells indicate an association of the CTD with the deacetylated histone tails of H3 and H4 that is necessary for the recruitment of Sir3. The efficiency of spreading depends upon the apparent stoichiometry and stability during the initiation event. The predicted Cdc6 domain III winged-helix structure may well be responsible for dimerization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.