ArticleMolecular and cellular biology2006
An hpr1 point mutation that impairs transcription and mRNP biogenesis without increasing recombination.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 40 citations in OpenAlex.
- Cellular imbalance of specific RNA-binding proteins associates with harmful R-loops.PLoS genetics · 2025Article
- R-loops at centromeric chromatin contribute to defects in kinetochore integrity and chromosomal instability in budding yeast.Molecular biology of the cell · 2021Article
- The polyadenosine RNA-binding protein ZC3H14 interacts with the THO complex and coordinately regulates the processing of neuronal transcripts.Nucleic acids research · 2018Article
- Review
- Structural and biochemical analyses of the DEAD-box ATPase Sub2 in association with THO or Yra1.eLife · 2017Article
- Excess of Yra1 RNA-Binding Factor Causes Transcription-Dependent Genome Instability, Replication Impairment and Telomere Shortening.PLoS genetics · 2016Article
- Nuclear pore components affect distinct stages of intron-containing gene expression.Nucleic acids research · 2015Article
- A genome-wide function of THSC/TREX-2 at active genes prevents transcription-replication collisions.Nucleic acids research · 2014Article
- Transcription and recombination: when RNA meets DNA.Cold Spring Harbor perspectives in biology · 2014Review
- The Thoc1 encoded ribonucleoprotein is a substrate for the NEDD4-1 E3 ubiquitin protein ligase.PloS one · 2013Article
- Architecture and nucleic acids recognition mechanism of the THO complex, an mRNP assembly factor.The EMBO journal · 2012Article
- Interference between DNA replication and transcription as a cause of genomic instability.Current genomics · 2012Article
- Topological constraints impair RNA polymerase II transcription and causes instability of plasmid-borne convergent genes.Nucleic acids research · 2012Article
- Genome-wide function of THO/TREX in active genes prevents R-loop-dependent replication obstacles.The EMBO journal · 2011Article
- New suppressors of THO mutations identify Thp3 (Ypr045c)-Csn12 as a protein complex involved in transcription elongation.Molecular and cellular biology · 2011Article
- The THO complex as a key mRNP biogenesis factor in development and cell differentiation.Journal of biology · 2010Article
- The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants.Molecular and cellular biology · 2009Article
- R-loops do not accumulate in transcription-defective hpr1-101 mutants: implications for the functional role of THO/TREX.Nucleic acids research · 2009Article
- Sem1 is a functional component of the nuclear pore complex-associated messenger RNA export machinery.The Journal of cell biology · 2009Article
- The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability.Molecular biology of the cell · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
THO/TREX, a conserved eukaryotic protein complex, is a key player at the interface between transcription and mRNP metabolism. The lack of a functional THO complex impairs transcription, leads to transcription-dependent hyperrecombination, causes mRNA export defects and fast mRNA decay, and retards replication fork progression in a transcription-dependent manner. To get more insight into the interconnection between mRNP biogenesis and genomic instability, we searched for HPR1 mutations that differentially affect gene expression and recombination. We isolated mutants that were barely affected in gene expression but exhibited a hyperrecombination phenotype. In addition, we isolated a mutant, hpr1-101, with a strong defect in transcription, as observed for lacZ, and a general defect in mRNA export that did not display a relevant hyperrecombination phenotype. In THO single-null mutants, but not in the hpr1 point mutants studied, THO and its subunits were unstable. Interestingly, in contrast to hyperrecombinant null mutants, hpr1-101 did not cause retardation of replication fork progression. Transcription and mRNP biogenesis can therefore be impaired by THO/TREX dysfunction without increasing recombination, suggesting that it is possible to separate the mechanism(s) responsible for mRNA biogenesis defects from the further step of triggering transcription-dependent recombination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.