ArticleTransgenic research2006
Expression of the cry9Aa2 B.t. gene in tobacco chloroplasts confers resistance to potato tuber moth.
Article in Transgenic research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Chloroplast Genome Engineering: A Plausible Approach to Combat Chili Thrips and Other Agronomic Insect Pests of Crops.Plants (Basel, Switzerland) · 2023Review
- An analysis of codon utilization patterns in the chloroplast genomes of three species of Coffea.BMC genomic data · 2023Article
- Chloroplast Engineering: Fundamental Insights and Its Application in Amelioration of Environmental Stress.Applied biochemistry and biotechnology · 2023Review
- Identification and validation of the wound and insect bite early inducible promoter from3 Biotech · 2022Article
- Advancing organelle genome transformation and editing for crop improvement.Plant communications · 2021Review
- Green giant-a tiny chloroplast genome with mighty power to produce high-value proteins: history and phylogeny.Plant biotechnology journal · 2021Review
- Mitigation of deleterious phenotypes in chloroplast-engineered plants accumulating high levels of foreign proteins.Biotechnology for biofuels · 2021Article
- Plastid Transformation: How Does it Work? Can it Be Applied to Crops? What Can it Offer?International journal of molecular sciences · 2020Review
- The plastid genome as a chassis for synthetic biology-enabled metabolic engineering: players in gene expression.Plant cell reports · 2018Review
- Efficient Plastid Transformation in Arabidopsis.Plant physiology · 2017Article
- Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts.Transgenic research · 2017Article
- Recent achievements obtained by chloroplast transformation.Plant methods · 2017Review
- Chloroplast genomes: diversity, evolution, and applications in genetic engineering.Genome biology · 2016Review
- In-Plant Protection againstFrontiers in plant science · 2016Article
- Optimization of transplastomic production of hemicellulases in tobacco: effects of expression cassette configuration and tobacco cultivar used as production platform on recombinant protein yields.Biotechnology for biofuels · 2013Article
- Plastid genetic engineering in Solanaceae.Protoplasma · 2012Review
- Detrimental effect of expression of Bt endotoxin Cry1Ac on in vitro regeneration, in vivo growth and development of tobacco and cotton transgenics.Journal of biosciences · 2011Article
- Plastid biotechnology: food, fuel, and medicine for the 21st century.Plant physiology · 2011Review
- Plant plastid engineering.Current genomics · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report here the control of potato tuber moth (Phthorimaea operculella) by incorporating a truncated Bacillus thuringiensis cry9Aa2 gene in the plastid genome. Plasmids pSKC84 and pSKC85 are derivatives of a new polycistronic plastid transformation vector, pPRV312L, that carries spectinomycin resistance (aadA) as a selective marker and targets insertions in the trnI-trnA intergenic region. The Cry9Aa2 N-terminal region (82.1 kDa; 734 amino acids) was expressed in a cassette, which consists of 49 nucleotides of the cry9Aa2 leader and the 3'-untranslated region of the plastid rbcL gene (TrbcL), and relies on readthrough transcription from the plastid rRNA operon. In a tobacco leaf bioassay, expression of Cry9Aa2 conferred resistance to potato tuber moth. In accordance, the Cry9Aa2 insecticidal protein accumulated to high levels, approximately 10% of the total soluble cellular protein and approximately 20% in the membrane fraction. However, high-level Cry9Aa2 expression significantly delayed plant development. Thus, a practical system to control potato tuber moth by Cry9Aa2 expression calls for down-regulation of its expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.