ArticleNature chemical biology2006
Biosynthesis of Dictyostelium discoideum differentiation-inducing factor by a hybrid type I fatty acid-type III polyketide synthase.
Article in Nature chemical biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed, 130 citations in OpenAlex.
- Enzymatic synthesis of bioactive quinolones and (thio)coumarins by fungal type III polyketide synthases.The FEBS journal · 2026Article
- Production of antibacterial compounds by a Steely hybrid polyketide synthase in Dictyostelium.FEBS open bio · 2026Article
- Serendipity and the Slime Mold: A Visual Survey of High-Molecular-Weight Protein Assemblies Reveals the Structure of the Polyketide Synthase Pks16.Molecular & cellular proteomics : MCP · 2026Article
- Molecular basis of very-long-chain fatty acid elongation by the CER6-GL2 enzyme complex in plant wax biosynthesis.Science advances · 2025Article
- Global profiling of polyketide synthases in facultative multicellular eukaryotes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Identification of Slime Mold Metabolites That Confer Protection to Commercial Crops against Root-Knot Nematodes.Journal of agricultural and food chemistry · 2025Article
- The Amoeba Dictyostelium discoideum as Novel Production Host for Complex Substances.Advances in biochemical engineering/biotechnology · 2025Review
- Isolation and Structure Determination of New Pyrones fromMolecules (Basel, Switzerland) · 2024Article
- Generating polyketide diversity inProceedings. Biological sciences · 2022Article
- Engineering the amoeba Dictyostelium discoideum for biosynthesis of a cannabinoid precursor and other polyketides.Nature biotechnology · 2022Article
- Evolution and Diversity of Assembly-Line Polyketide Synthases.Chemical reviews · 2019Review
- 4-Methyl-5-Pentylbenzene-1,3-Diol Regulates Chemotactic Cell Aggregation and Spore Maturation Via Different Mechanisms in Dictyostelium discoideum.Current microbiology · 2019Article
- An individual-level selection model for the apparent altruism exhibited by cellular slime moulds.Journal of biosciences · 2018Article
- Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse.Chemical reviews · 2017Review
- Structural basis of head to head polyketide fusion by CorB.Chemical science · 2015Article
- Assembly line termination in cylindrocyclophane biosynthesis: discovery of an editing type II thioesterase domain in a type I polyketide synthase.Chemical science · 2015Article
- c-di-GMP induction of Dictyostelium cell death requires the polyketide DIF-1.Molecular biology of the cell · 2015Article
- Comparative genome and transcriptome analyses of the social amoeba Acytostelium subglobosum that accomplishes multicellular development without germ-soma differentiation.BMC genomics · 2015Article
- Nostopeptolide plays a governing role during cellular differentiation of the symbiotic cyanobacterium Nostoc punctiforme.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Cell signaling during development of Dictyostelium.Developmental biology · 2014Review
Corrections and comments
- Commented on by
Authors and funding
8 authors at 6 institutions in 3 countries.
Funding
Abstract
Differentiation-inducing factors (DIFs) are well known to modulate formation of distinct communal cell types from identical Dictyostelium discoideum amoebas, but DIF biosynthesis remains obscure. We report complimentary in vivo and in vitro experiments identifying one of two approximately 3,000-residue D. discoideum proteins, termed 'steely', as responsible for biosynthesis of the DIF acylphloroglucinol scaffold. Steely proteins possess six catalytic domains homologous to metazoan type I fatty acid synthases (FASs) but feature an iterative type III polyketide synthase (PKS) in place of the expected FAS C-terminal thioesterase used to off load fatty acid products. This new domain arrangement likely facilitates covalent transfer of steely N-terminal acyl products directly to the C-terminal type III PKS active sites, which catalyze both iterative polyketide extension and cyclization. The crystal structure of a steely C-terminal domain confirms conservation of the homodimeric type III PKS fold. These findings suggest new bioengineering strategies for expanding the scope of fatty acid and polyketide biosynthesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.