ArticleEMBO reports2006
A novel human artificial chromosome gene expression system using herpes simplex virus type 1 vectors.
Article in EMBO reports, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Non-replicative herpes simplex virus genomic and amplicon vectors for gene therapy - an update.Gene therapy · 2025Review
- Co-infection of HSV-1 amplicons containing theBiochemistry and biophysics reports · 2024Article
- Pluripotent stem cell-based gene therapy approach: human de novo synthesized chromosomes.Cellular and molecular life sciences : CMLS · 2021Review
- Gene expressing human artificial chromosome vectors: Advantages and challenges for gene therapy.Experimental cell research · 2020Review
- Multigene human artificial chromosome vector delivery with herpes simplex virus 1 amplicons.Experimental cell research · 2020Article
- Review
- A pathway from chromosome transfer to engineering resulting in human and mouse artificial chromosomes for a variety of applications to bio-medical challenges.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Developing de novo human artificial chromosomes in embryonic stem cells using HSV-1 amplicon technology.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Replication of alpha-satellite DNA arrays in endogenous human centromeric regions and in human artificial chromosome.Nucleic acids research · 2014Article
- Comparative study of artificial chromosome centromeres in human and murine cells.European journal of human genetics : EJHG · 2013Article
- A new generation of human artificial chromosomes for functional genomics and gene therapy.Cellular and molecular life sciences : CMLS · 2013Review
- Organization of synthetic alphoid DNA array in human artificial chromosome (HAC) with a conditional centromere.ACS synthetic biology · 2012Article
- Evolutionary insights into the role of the essential centromere protein CAL1 in Drosophila.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- Breaking the HAC Barrier: histone H3K9 acetyl/methyl balance regulates CENP-A assembly.The EMBO journal · 2012Article
- Human artificial chromosome (HAC) vector with a conditional centromere for correction of genetic deficiencies in human cells.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Human artificial chromosomes for gene delivery and the development of animal models.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Review
- Functional human artificial chromosomes are generated and stably maintained in human embryonic stem cells.Human molecular genetics · 2011Article
- Genetic therapy for the nervous system.Human molecular genetics · 2011Review
- Constitutive and Inducible Innate Responses in Cells Infected by HSV-1-Derived Amplicon Vectors.The open virology journal · 2010Article
- Herpes simplex virus type 1/adeno-associated virus hybrid vectors.The open virology journal · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human artificial chromosome (HAC) vectors are an important gene transfer system for expression and complementation studies. We describe a significant advance in HAC technology using infectious herpes simplex virus type 1 (HSV-1) amplicon vectors for delivery. This highly efficient method has allowed gene-expressing HACs to be established in glioma-, kidney- and lung-derived cells. We also developed an HSV-1 hypoxanthine phosphoribosyltransferase (HPRT) HAC vector, which generated functional HPRT-expressing HACs that complemented the genetic deficiency in human cells. The transduction efficiency of the HSV-1 HAC amplicons is several orders of magnitude higher than lipofection-mediated delivery. Studies on HAC stability between cell types showed important differences that have implications for HAC development and gene expression in human cells. This is the first report of establishing gene-expressing HACs in human cells by using an efficient, high-capacity viral vector and by identifying factors that are involved in cell-type-specific HAC instability. The work is a significant advance for HAC technology and the development of HAC gene expression systems in human cells.
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