ArticlePlant physiology2006
Quantification of plasmodesmatal endoplasmic reticulum coupling between sieve elements and companion cells using fluorescence redistribution after photobleaching.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Immunohistochemical Localization of Proteins in the Phloem: Problems and Solutions.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Measuring Phloem Transport Velocity on a Tissue-Level Using a Phloem-Mobile Dye.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Live-Cell Imaging of Fluorescently-Tagged Phloem Proteins with Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- OsDISMO1: A Novel Transporter for Molybdenum Distribution in Rice Shoots.Rice (New York, N.Y.) · 2025Article
- The sieve-element endoplasmic reticulum: A focal point of phytoplasma-host plant interaction?Frontiers in microbiology · 2023Article
- Article
- Advances in Synthetic Fluorescent Probe Labeling for Live-Cell Imaging in Plants.Plant & cell physiology · 2021Review
- Intercellular trafficking via plasmodesmata: molecular layers of complexity.Cellular and molecular life sciences : CMLS · 2021Review
- The MCTP-SNARE Complex Regulates Florigen Transport in Arabidopsis.The Plant cell · 2019Article
- Direct Comparison of Leaf Plasmodesma Structure and Function in Relation to Phloem-Loading Type.Plant physiology · 2019Article
- The plant membrane surrounding powdery mildew haustoria shares properties with the endoplasmic reticulum membrane.Journal of experimental botany · 2017Article
- Structure, phylogeny, allelic haplotypes and expression of sucrose transporter gene families in Saccharum.BMC genomics · 2016Article
- Putting the Squeeze on Plasmodesmata: A Role for Reticulons in Primary Plasmodesmata Formation.Plant physiology · 2015Article
- Article
- Plasmodesmata without callose and calreticulin in higher plants - open channels for fast symplastic transport?Frontiers in plant science · 2014Article
- How phloem-feeding insects face the challenge of phloem-located defenses.Frontiers in plant science · 2013Review
- Increased expression of a phloem membrane protein encoded by NHL26 alters phloem export and sugar partitioning in Arabidopsis.The Plant cell · 2013Article
- Receptor-mediated signaling at plasmodesmata.Frontiers in plant science · 2013Article
- The quest for four-dimensional imaging in plant cell biology: it's just a matter of time.Annals of botany · 2012Review
- Identification of lipids and lipid-binding proteins in phloem exudates from Arabidopsis thaliana.Journal of experimental botany · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgenic tobacco (Nicotiana tabacum) was studied to localize the activity of phloem loading during development and to establish whether the endoplasmic reticulum (ER) of the companion cell (CC) and the sieve element (SE) reticulum is continuous by using a SUC2 promoter-green fluorescent protein (GFP) construct targeted to the CC-ER. Expression of GFP marked the collection phloem in source leaves and cotyledons as expected, but also the transport phloem in stems, petioles, midveins of sink leaves, nonphotosynthetic flower parts, roots, and newly germinated seedlings, suggesting that sucrose retrieval along the pathway is an integral component of phloem function. GFP fluorescence was limited to CCs where it was visualized as a well-developed ER network in close proximity to the plasma membrane. ER coupling between CC and SEs was tested in wild-type tobacco using an ER-specific fluorochrome and fluorescence redistribution after photobleaching (FRAP), and showed that the ER is continuous via pore-plasmodesma units. ER coupling between CC and SE was quantified by determining the mobile fraction and half-life of fluorescence redistribution and compared with that of other cell types. In all tissues, fluorescence recovered slowly when it was rate limited by plasmodesmata, contrasting with fast intracellular FRAP. FRAP was unaffected by treatment with cytochalasin D. The highest degree of ER coupling was measured between CC and SE. Intimate ER coupling is consistent with a possible role for ER in membrane protein and signal exchange between CC and SE. However, a complete lack of GFP transfer between CC and SE indicated that the intraluminal pore-plasmodesma contact has a size exclusion limit below 27 kD.
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