ArticleThe Plant cell2006
Tobacco WLIM1 is a novel F-actin binding protein involved in actin cytoskeleton remodeling.
Article in The Plant cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 91 citations in OpenAlex.
- Identification and characterization of LIM family genes in Tartary buckwheat: insights into functional divergence and abiotic stress responses.BMC plant biology · 2026Article
- Heat-stable protein PGSL1 enhances pollen germination and tube growth at high temperature.Nature communications · 2025Article
- Arabidopsis Actin-Binding Protein WLIM2A Links PAMP-Triggered Immunity and Cytoskeleton Organization.International journal of molecular sciences · 2024Article
- Exploring the Role of the Plant Actin Cytoskeleton: From Signaling to Cellular Functions.International journal of molecular sciences · 2023Review
- A genome‑wide approach to the systematic and comprehensive analysis of LIM gene family in sorghum (Sorghum bicolor L.).Genomics & informatics · 2023Article
- ETHYLENE RESPONSE FACTOR 34 promotes secondary cell wall thickening and strength of rice peduncles.Plant physiology · 2022Article
- Silencing of a Cotton Actin-Binding Protein GhWLIM1C Decreases Resistance againstPlants (Basel, Switzerland) · 2022Article
- Microtubules play a crucial role in regulating actin organization and cell initiation in cotton fibers.Plant cell reports · 2022Article
- Phosphatidylinositol 3-phosphate regulates SCAB1-mediated F-actin reorganization during stomatal closure in Arabidopsis.The Plant cell · 2022Article
- LIM domain proteins in cell mechanobiology.Cytoskeleton (Hoboken, N.J.) · 2021Review
- Genome-wide identification, characterization and expression analysis of the LIM transcription factor family in quinoa.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Article
- The Balance between Actin-Bundling Factors Controls Actin Architecture in Pollen Tubes.iScience · 2019Article
- Actin Cytoskeleton as Actor in Upstream and Downstream of Calcium Signaling in Plant Cells.International journal of molecular sciences · 2019Review
- Genome-Wide Analysis ofInternational journal of molecular sciences · 2019Article
- Actin Bundles in The Pollen Tube.International journal of molecular sciences · 2018Review
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- Article
- Review
- CRP2, a new invadopodia actin bundling factor critically promotes breast cancer cell invasion and metastasis.Oncotarget · 2016Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used confocal microscopy and in vitro analyses to show that Nicotiana tabacum WLIM1, a LIM domain protein related to animal Cys-rich proteins, is a novel actin binding protein in plants. Green fluorescent protein (GFP)-tagged WLIM1 protein accumulated in the nucleus and cytoplasm of tobacco BY2 cells. It associated predominantly with actin cytoskeleton, as demonstrated by colabeling and treatment with actin-depolymerizing latrunculin B. High-speed cosedimentation assays revealed the ability of WLIM1 to bind directly to actin filaments with high affinity. Fluorescence recovery after photobleaching and fluorescence loss in photobleaching showed a highly dynamic in vivo interaction of WLIM1-GFP with actin filaments. Expression of WLIM1-GFP in BY2 cells significantly delayed depolymerization of the actin cytoskeleton induced by latrunculin B treatment. WLIM1 also stabilized actin filaments in vitro. Importantly, expression of WLIM1-GFP in Nicotiana benthamiana leaves induces significant changes in actin cytoskeleton organization, specifically, fewer and thicker actin bundles than in control cells, suggesting that WLIM1 functions as an actin bundling protein. This hypothesis was confirmed by low-speed cosedimentation assays and direct observation of F-actin bundles that formed in vitro in the presence of WLIM1. Taken together, these data identify WLIM1 as a novel actin binding protein that increases actin cytoskeleton stability by promoting bundling of actin filaments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.