ArticleBiophysical journal2006
High-resolution AFM of membrane proteins directly incorporated at high density in planar lipid bilayer.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 118 citations in OpenAlex.
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- γ-Secretase Partitioning into Lipid Bilayers Remodels Membrane Microdomains after Direct Insertion.Langmuir : the ACS journal of surfaces and colloids · 2020Article
- Atomic Force Microscopy Meets Biophysics, Bioengineering, Chemistry, and Materials Science.ChemSusChem · 2019Review
- Properties of Omp2a-Based Supported Lipid Bilayers: Comparison with Polymeric Bioinspired Membranes.ACS omega · 2018Article
- Lipid-Detergent Phase Transitions During Detergent-Mediated Liposome Solubilization.The Journal of membrane biology · 2016Article
- H2 -Fueled ATP Synthesis on an Electrode: Mimicking Cellular Respiration.Angewandte Chemie (International ed. in English) · 2016Article
- Insertion and self-diffusion of a monotopic protein, the Aquifex aeolicus sulfide quinone reductase, in supported lipid bilayers.The European physical journal. E, Soft matter · 2015Article
- Bioinspired membrane-based systems for a physical approach of cell organization and dynamics: usefulness and limitations.Interface focus · 2015Review
- Construction of P-glycoprotein incorporated tethered lipid bilayer membranes.Biochemistry and biophysics reports · 2015Article
- Ligand binding alters dimerization and sequestering of urokinase receptors in raft-mimicking lipid mixtures.Biophysical journal · 2014Article
- Single lipid extraction: the anchoring strength of cholesterol in liquid-ordered and liquid-disordered phases.Biophysical journal · 2014Article
- Detergent-mediated incorporation of transmembrane proteins in giant unilamellar vesicles with controlled physiological contents.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The nanomechanical properties of lipid membranes are significantly influenced by the presence of ethanol.Biophysical journal · 2013Article
- Lipid nanotechnology.International journal of molecular sciences · 2013Article
- The open gate structure of the membrane-embedded KcsA potassium channel viewed from the cytoplasmic side.Scientific reports · 2013Article
- Beyond Saffman-Delbruck approximation: a new regime for 2D diffusion of α-hemolysin complexes in supported lipid bilayer.The European physical journal. E, Soft matter · 2012Article
- Molecular interactions of proteins and peptides at interfaces studied by sum frequency generation vibrational spectroscopy.Langmuir : the ACS journal of surfaces and colloids · 2012Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The heterologous expression and purification of membrane proteins represent major limitations for their functional and structural analysis. Here we describe a new method of incorporation of transmembrane proteins in planar lipid bilayer starting from 1 pmol of solubilized proteins. The principle relies on the direct incorporation of solubilized proteins into a preformed planar lipid bilayer destabilized by dodecyl-beta-maltoside or dodecyl-beta-thiomaltoside, two detergents widely used in membrane biochemistry. Successful incorporations are reported at 20 degrees C and at 4 degrees C with three bacterial photosynthetic multi-subunit membrane proteins. Height measurements by atomic force microscopy (AFM) of the extramembraneous domains protruding from the bilayer demonstrate that proteins are unidirectionally incorporated within the lipid bilayer through their more hydrophobic domains. Proteins are incorporated at high density into the bilayer and on incubation diffuse and segregate into protein close-packing areas. The high protein density allows high-resolution AFM topographs to be recorded and protein subunits organization delineated. This approach provides an alternative experimental platform to the classical methods of two-dimensional crystallization of membrane proteins for the structural analysis by AFM. Furthermore, the versatility and simplicity of the method are important intrinsic properties for the conception of biosensors and nanobiomaterials involving membrane proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.