ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2006
Ty3/gypsy-like retrotransposon knockout of a 2-methyl-6-phytyl-1,4-benzoquinone methyltransferase is non-lethal, uncovers a cryptic paralogous mutation, and produces novel tocopherol (vitamin E) profiles in sunflower.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 49 citations in OpenAlex.
- Genetic mapping of loci involved in oil tocopherol composition control in Russian sunflower (Helianthus annuus L.) lines.G3 (Bethesda, Md.) · 2022Article
- Contribution of the Ratio of Tocopherol Homologs to the Oxidative Stability of Commercial Vegetable Oils.Molecules (Basel, Switzerland) · 2018Article
- Genotypic and Environmental Effects on Tocopherol Content in Almond.Antioxidants (Basel, Switzerland) · 2018Review
- Vitamin E Biosynthesis and Its Regulation in Plants.Antioxidants (Basel, Switzerland) · 2017Review
- Novel Loci Underlie Natural Variation in Vitamin E Levels in Maize Grain.The Plant cell · 2017Article
- Genome-wide association study and pathway-level analysis of tocochromanol levels in maize grain.G3 (Bethesda, Md.) · 2013Article
- Positional cloning of a candidate gene for resistance to the sunflower downy mildew, Plasmopara halstedii race 300.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Identification, characterization and distribution of transposable elements in the flax (Linum usitatissimum L.) genome.BMC genomics · 2012Article
- Isolation, characterization, and structure analysis of a non-TIR-NBS-LRR encoding candidate gene from MYMIV-resistant Vigna mungo.Molecular biotechnology · 2012Article
- Genetic basis of unstable expression of high gamma-tocopherol content in sunflower seeds.BMC plant biology · 2012Article
- An analysis of sequence variability in eight genes putatively involved in drought response in sunflower (Helianthus annuus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- SSRs and INDELs mined from the sunflower EST database: abundance, polymorphisms, and cross-taxa utility.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
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- Review
- Three non-allelic epistatically interacting methyltransferase mutations produce novel tocopherol (vitamin E) profiles in sunflower.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
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3 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The m (Tph(1)) mutation partially disrupts the synthesis of alpha-tocopherol (vitamin E) in sunflower (Helianthus annuus L.) seeds and was predicted to disrupt a methyltransferase activity necessary for the synthesis of alpha- and gamma-tocopherol. We identified and isolated two 2-methyl-6-phytyl-1,4-benzoquinone/2-methyl-6-solanyl-1,4-benzoquinone methyltransferase (MPBQ/MSBQ-MT) paralogs from sunflower (MT-1 and MT-2), resequenced MT-1 and MT-2 alleles from wildtype (m(+) m(+)) and mutant (m m) inbred lines, identified m as a non-lethal knockout mutation of MT-1 caused by the insertion of a 5.2 kb Ty3/gypsy-like retrotransposon in exon 1, and uncovered a cryptic codominant mutation (d) in a wildtype x mutant F(2) population predicted to be segregating for the m mutation only. MT-1 and m cosegregated and mapped to linkage group 1 and MT-1 was not transcribed in mutant homozygotes (m m). The m locus was epistatic to the d locus--the d locus had no effect in m(+) m(+) and m(+) m individuals, but significantly increased beta-tocopherol percentages in m m individuals. MT-2 and d cosegregated, MT-2 alleles isolated from mutant homozygotes (d d) carried a 30 bp insertion at the start of the 5'-UTR, and MT-2 was more strongly transcribed in seeds and leaves of wildtype (d(+) d(+)) than mutant (d d) homozygotes (transcripts were 2.2- to 5.0-fold more abundant in the former than the latter). The double mutant (m m d d) was non-lethal and produced 24-45% alpha- and 55-74% beta-tocopherol (the wildtype produced 96% alpha- and 4% beta-tocopherol). MT-2 compensated for the loss of the MT-1 function, and the MT-2 mutation profoundly affected the synthesis of tocopherols without adversely affecting the synthesis of plastoquinone crucial for normal plant growth and development.
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