ArticleThe Histochemical journal1990
Immunocytochemical detection of peroxisomal beta-oxidation enzymes in cryostat and paraffin sections of human post mortem liver.
Article in The Histochemical journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- A brief history of the human liver peroxisome.Histochemistry and cell biology · 2026Review
- Isolated dihydroxyacetonephosphate-acyl-transferase deficiency in rhizomelic chondrodysplasia punctata: clinical presentation, metabolic and histological findings.European journal of pediatrics · 1996Article
- In situ heterogeneity of peroxisomal oxidase activities: an update.The Histochemical journal · 1996Review
- The importance of tissue fixation for light microscopic immunohistochemical localization of peroxisomal proteins: the superiority of Carnoy's fixative over Baker's formalin and Bouin's solution.Histochemistry and cell biology · 1995Article
- Immunoblot analysis of peroxisomal proteins in liver and fibroblasts from patients.Journal of inherited metabolic disease · 1995Article
- Immunocytochemical localization of peroxisomal proteins in human liver and kidney.Journal of inherited metabolic disease · 1995Article
- Secondary alterations of human hepatocellular peroxisomes.Journal of inherited metabolic disease · 1995Review
- Morphometry of peroxisomes and immunolocalization of peroxisomal proteins in the liver of patients with generalised peroxisomal disorders.Virchows Archiv. A, Pathological anatomy and histopathology · 1993Article
- X-linked recessive chondrodysplasia punctata with XY translocation in a stillborn fetus.Human genetics · 1991Article
- Article
- The role of histochemical investigations in metabolic disorders affecting the liver.Journal of inherited metabolic disease · 1991Review
- Peroxisomal localization of the immunoreactive beta-oxidation enzymes in a neonate with a beta-oxidation defect. Pathological observations in liver, adrenal cortex and kidney.Virchows Archiv. A, Pathological anatomy and histopathology · 1991Article
- Liver pathology and immunocytochemistry in congenital peroxisomal diseases: a review.Journal of inherited metabolic disease · 1991Review
- Post-mortem visualization of peroxisomes in rat and in human liver.The Histochemical journal · 1990Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The immunocytochemical visualization of the peroxisomal beta-oxidation enzymes was investigated in three human post mortem liver samples. Acyl-CoA oxidase, bifunctional protein and 3-oxoacyl-CoA thiolase remained immunocytochemically detectable 30, 55 and 72 h after death. Peroxisomes in the parenchymal cells were clearly visualized for light microscopy (paraffin and cryostat sections), using protein A-gold in combination with silver enhancement. In two samples catalase activity became very weak, but catalase antigenicity was well preserved. The findings prove the diagnostic value of post mortem samples, even after extreme conditions of tissue conservation. The technique of immunocytochemical staining for the peroxisomal beta-oxidation enzymes on unmounted cryostat sections has not been reported previously. This method allows a quick diagnosis of biopsies from patients suspected of peroxisomal disorders.
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