ArticleMolecular biology of the cell2006
Top3 processes recombination intermediates and modulates checkpoint activity after DNA damage.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity.Nature communications · 2020Article
- Review
- A novel method of using Deep Belief Networks and genetic perturbation data to search for yeast signaling pathways.PloS one · 2018Article
- DNA replication stress: from molecular mechanisms to human disease.Chromosoma · 2017Review
- Top3-Rmi1 DNA single-strand decatenase is integral to the formation and resolution of meiotic recombination intermediates.Molecular cell · 2015Article
- The dissolution of double Holliday junctions.Cold Spring Harbor perspectives in biology · 2014Review
- Resolution by unassisted Top3 points to template switch recombination intermediates during DNA replication.The Journal of biological chemistry · 2013Article
- Epistasis analysis between homologous recombination genes in Saccharomyces cerevisiae identifies multiple repair pathways for Sgs1, Mus81-Mms4 and RNase H2.Mutation research · 2011Article
- Pathways for Holliday junction processing during homologous recombination in Saccharomyces cerevisiae.Molecular and cellular biology · 2011Article
- Holliday junction-containing DNA structures persist in cells lacking Sgs1 or Top3 following exposure to DNA damage.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Identification of Trypanosoma brucei RMI1/BLAP75 homologue and its roles in antigenic variation.PloS one · 2011Article
- RNase-dependent discontinuities associated with the crossovers of spontaneously formed joint DNA molecules in Physarum polycephalum.Chromosoma · 2010Article
- Replication and recombination factors contributing to recombination-dependent bypass of DNA lesions by template switch.PLoS genetics · 2010Article
- The roles of the Saccharomyces cerevisiae RecQ helicase SGS1 in meiotic genome surveillance.PloS one · 2010Article
- TOPO3alpha influences antigenic variation by monitoring expression-site-associated VSG switching in Trypanosoma brucei.PLoS pathogens · 2010Article
- Maintaining genome stability at the replication fork.Nature reviews. Molecular cell biology · 2010Review
- Timing is everything: cell cycle control of Rad52.Cell division · 2010Article
- Esc2 and Sgs1 act in functionally distinct branches of the homologous recombination repair pathway in Saccharomyces cerevisiae.Molecular biology of the cell · 2009Article
- The genetic consequences of ablating helicase activity and the Top3 interaction domain of Sgs1.DNA repair · 2008Article
- Defective p53 engagement after the induction of DNA damage in cells deficient in topoisomerase 3beta.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutation of TOP3 in Saccharomyces cerevisiae causes poor growth, hyperrecombination, and a failure to fully activate DNA damage checkpoints in S phase. Here, we report that overexpression of a dominant-negative allele of TOP3, TOP3(Y356F), which lacks the catalytic (decatenation) activity of Top3, causes impaired S-phase progression and the persistence of abnormal DNA structures (X-shaped DNA molecules) after exposure to methylmethanesulfonate. The impaired S-phase progression is due to a persistent checkpoint-mediated cell cycle delay and can be overridden by addition of caffeine. Hence, the catalytic activity of Top3 is not required for DNA damage checkpoint activation, but it is required for normal S-phase progression after DNA damage. We also present evidence that the checkpoint-mediated cell cycle delay and persistence of X-shaped DNA molecules resulting from overexpression of TOP3(Y356F) are downstream of Rad51 function. We propose that Top3 functions in S phase to both process homologous recombination intermediates and modulate checkpoint activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.