ArticlePlant molecular biology2006
The Arabidopsis Bio2 protein requires mitochondrial targeting for activity.
Article in Plant molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Genomes of NBMC genomics · 2026Article
- Physiological and Transcriptomic Analyses Reveal the Mechanisms Underlying Methyl Jasmonate-Induced Mannitol Stress Resistance in Banana.Plants (Basel, Switzerland) · 2024Article
- Biotin Auxotrophy and Biotin Enhanced Germ Tube Formation in Candida albicans.Microorganisms · 2016Article
- Expanding functional repertoires of fungal peroxisomes: contribution to growth and survival processes.Frontiers in physiology · 2013Article
- A newly discovered function of peroxisomes: involvement in biotin biosynthesis.Plant signaling & behavior · 2012Review
- Article
- Peroxisomes are involved in biotin biosynthesis in Aspergillus and Arabidopsis.The Journal of biological chemistry · 2011Article
- Characterization of Raphanus sativus pentatricopeptide repeat proteins encoded by the fertility restorer locus for Ogura cytoplasmic male sterility.The Plant cell · 2008Article
- Mitochondrial biogenesis and function in Arabidopsis.The arabidopsis book · 2008Article
- A bifunctional locus (BIO3-BIO1) required for biotin biosynthesis in Arabidopsis.Plant physiology · 2008Article
- The role of plant mitochondria in the biosynthesis of coenzymes.Photosynthesis research · 2007Review
- Biotin uptake in prokaryotes by solute transporters with an optional ATP-binding cassette-containing module.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondria are involved in the production of various vitamins, such as biotin, in plants. It is unclear why these biosynthetic pathways have been maintained partly or entirely within the mitochondria throughout evolution. The last step in biotin biosynthesis occurs within the mitochondria and is catalyzed by the biotin synthase complex containing the BIO2 gene product. We investigated whether the Arabidopsis Bio2 enzyme could function outside mitochondria, by trying to complement a bio2 mutant with a truncated version of BIO2 lacking the region encoding the mitochondrial targeting sequence. We describe the characterization of a new T-DNA allele of bio2, with the sole phenotype of an absence of biotin production, in contrast to the previously characterized EMS bio2 allele (Patton et al. 1998, Plant Physiol 116(3):935-946). We found that a cytosolic version of the Bio2 protein could not complement this mutant. Supplementation with the substrate dethiobiotin (DTB) also failed to rescue the mutant phenotype. Thus, the lack of availability of DTB in the cytosol is not the only factor preventing this reaction from occurring outside mitochondria. Bio2 requires mitochondrial targeting for activity, enabling it to fulfill its role in biotin synthesis. The reaction catalyzed by Bio2 may be subject to biochemical constraints, and the apparent close connection with the mitochondrial Fe-S machinery may account for the reaction being retained within the organelle.
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