ArticlePlant molecular biology2006
Global analysis of gene expression during development and ripening of citrus fruit flesh. A proposed mechanism for citric Acid utilization.
Article in Plant molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 192 citations in OpenAlex.
- Molecular orchestration of malate and citrate metabolism: regulatory networks governing organic acid dynamics and fruit quality attributes.Horticulture research · 2026Article
- Study on the differences in sugar-acid metabolism of Sweet Orange 'Newhall' with different rootstocks.BMC plant biology · 2025Article
- OsJAZ10 Modulates Metabolite Profiles in Rice Seedlings in Response to Alkaline Stress.Rice (New York, N.Y.) · 2025Article
- Phytochemicals, Organic Acid, and Vitamins in Red Rhapsody Strawberry-Content and Storage Stability.Foods (Basel, Switzerland) · 2025Article
- Untargeted Metabolomics Comparison and Nutrition Evaluation of Geographical Indication Newhall Navel Oranges in China.Foods (Basel, Switzerland) · 2025Article
- ATP Citrate LyasePlants (Basel, Switzerland) · 2024Article
- NMR metabolomics as a complementary tool to brix-acid tests for navel orange quality control of long-term cold storage.Scientific reports · 2024Article
- Dynamic Analysis of the Fruit Sugar-Acid Profile in a Fresh-Sweet Mutant and Wild Type in 'Shatangju' (Plants (Basel, Switzerland) · 2024Article
- A natural mutation in the promoter of the aconitase geneHorticulture research · 2024Article
- Gamma-Aminobutyric Acid Accumulation Contributes toPlants (Basel, Switzerland) · 2023Article
- Effects of Exogenous Application of Glycine Betaine Treatment on 'Huangguoggan' Fruit during Postharvest Storage.International journal of molecular sciences · 2023Article
- Article
- GABA shunt pathway is stimulated in response to early defoliation-induced carbohydrate limitation in Mandarin fruits.Heliyon · 2023Article
- Comparative Analysis of the Response to Polyethylene Glycol-Simulated Drought Stress in Roots from Seedlings of "Modern" and "Ancient" Wheat Varieties.Plants (Basel, Switzerland) · 2023Article
- Metabolomics and genetics of reproductive bud development inFrontiers in plant science · 2023Article
- Metabolomic and transcriptomic analyses reveal the effects of grafting on blood orange quality.Frontiers in plant science · 2023Article
- Comparative transcriptomics of wild and commercial Citrus during early ripening reveals how domestication shaped fruit gene expression.BMC plant biology · 2022Article
- Sugar and acid profile of loquat (Frontiers in plant science · 2022Article
- Transcriptome analysis of the pulp of citrus fruitlets suggests that domestication enhanced growth processes and reduced chemical defenses increasing palatability.Frontiers in plant science · 2022Article
- Changes and response mechanism of sugar and organic acids in fruits under water deficit stress.PeerJ · 2022Review
16 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microarrays of cDNA have been used to examine expression changes of 7000 genes during development and ripening of the fruit flesh of self-incompatible Citrus clementina, a non-climateric species. The data indicated that 2243 putative unigenes showed significant expression changes. Functional classification revealed that genes encoding for regulatory proteins were significantly overrepresented in the up-regulated gene clusters. The transcriptomic study together with the analyses of selected metabolites highlighted key physiological processes occurring during citrus fruit development and ripening such as water accumulation, carbohydrate build-up, acid reduction, pigment substitutions (carotenoid accumulation and chlorophyll decreases) and ascorbic acid diminution. Often, the combined analyses strongly suggested prevalence of specific metabolic alternatives. This observation has been exemplified with the proposal for a mechanism for citrate utilization, a process of much importance in citrus industry. Microarray data validated by real-time RT-PCR suggested that citrate was sequentially metabolyzed to isocitrate, 2-oxoglutarate and glutamate. Thereafter, glutamate was both utilized for glutamine production and catabolyzed through the gamma-aminobutirate (GABA) shunt (GABA --> succinate semialdehyde --> succinate). This last observation appears to be of special relevance since it links the proton consuming reaction glutamate + H(+)--> GABA + CO(2) with high acid levels. GG-MS determinations showed that glutamate was constant while GABA levels decreased at ripening in agreement with a feasible activation of the GABA shunt during acid catabolism. This suggestion provides a convincing explanation for the strong reduction of both citrate and cytoplasmatic acidity that takes place in citrus fruit flesh during development and ripening.
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