ArticleDiabetologia2006
Stimulation of cAMP signalling allows isolation of clonal pancreatic precursor cells from adult mouse pancreas.
Article in Diabetologia, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Type 1 diabetes and engineering enhanced islet transplantation.Advanced drug delivery reviews · 2022Review
- Induction of Expandable Tissue-Specific Progenitor Cells from Human Pancreatic Tissue through Transient Expression of Defined Factors.Molecular therapy. Methods & clinical development · 2019Article
- Characterization of induced tissue-specific stem cells from pancreas by a synthetic self-replicative RNA.Scientific reports · 2018Article
- Induced Tissue-Specific Stem Cells and Epigenetic Memory in Induced Pluripotent Stem Cells.International journal of molecular sciences · 2018Review
- β-Cell regeneration through the transdifferentiation of pancreatic cells: Pancreatic progenitor cells in the pancreas.Journal of diabetes investigation · 2016Review
- Induction of tissue-specific stem cells by reprogramming factors, and tissue-specific selection.Cell death and differentiation · 2015Article
- A new method for generating insulin-secreting cells from human pancreatic epithelial cells after islet isolation transformed by NeuroD1.Human gene therapy methods · 2014Article
- Establishment of a pancreatic stem cell line from fibroblast-derived induced pluripotent stem cells.Biomedical engineering online · 2014Article
- Culture Conditions for Mouse Pancreatic Stem Cells.Cell medicine · 2013Article
- Isolation Efficiency of Mouse Pancreatic Stem Cells Is Age Dependent.Cell medicine · 2013Article
- Analysis of the transcription factor cascade that induces endocrine and exocrine cell lineages from pancreatic progenitor cells using a polyoma-based episomal vector system.Journal of diabetes investigation · 2012Article
- Acinar-to-ductal metaplasia induced by adenovirus-mediated pancreatic expression of Isl1.PloS one · 2012Article
- Pancreatic stem/progenitor cells for the treatment of diabetes.The review of diabetic studies : RDS · 2010Review
- Recent advances in stem cell research for the treatment of diabetes.World journal of stem cells · 2009Article
- Identification and characterization of a novel expandable adult stem/progenitor cell population in the human exocrine pancreas.Journal of endocrinological investigation · 2008Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisDuct cells of the pancreas are thought to include latent progenitors of islet endocrine cells that can be induced to differentiate by appropriate morphogens. Here we developed a method for isolating pancreatic ductal epithelial cells from adult mice that overcomes the shortcomings of previous methods. MATERIALS AND
methodsPancreatic ductal cells were grown in serum-free DMEM/F12 medium in the presence of cholera toxin or 8-bromo-cyclic adenosine monophosphate, which is known to be an intracellular cAMP generator. Single cell cloning was performed by limiting dilution in serum-free medium.
resultsThe isolated clonal cells expressed high levels of cytokeratin and Ipf1 (formerly known as Pdx-1). Adenovirus-mediated expression of ngn3 (also known as Neurog3) and Ptf1a in these cells induced expression of insulin and somatostatin, and of carboxypeptidase A, respectively. Furthermore, albumin production was induced by dexamethasone or by long-term culture in serum-containing medium. CONCLUSIONS/
interpretationStimulation of the cAMP-dependent signalling allowed us to isolate clonal pancreatic ductal cells from adult mice. These cells are able to partially differentiate into endocrine cells, exocrine cells and hepatocyte-like cells and are therefore considered to have the characteristics of endodermal progenitor cells.
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