ArticleMarine biotechnology (New York, N.Y.)
New resources for marine genomics: bacterial artificial chromosome libraries for the Eastern and Pacific oysters (Crassostrea virginica and C. gigas).
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Sticky problems: extraction of nucleic acids from molluscs.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- Establishment of an Efficient and Flexible Genetic Manipulation Platform Based on a Fosmid Library for Rapid Generation of Recombinant Pseudorabies Virus.Frontiers in microbiology · 2018Article
- Comparative immunogenomics of molluscs.Developmental and comparative immunology · 2017Review
- Phylogenetic Analysis of Molluscan Metallothioneins: Evolutionary Insight from Crassostrea virginica.Journal of molecular evolution · 2016Article
- Construction of the BAC Library of Small Abalone (Haliotis diversicolor) for Gene Screening and Genome Characterization.Marine biotechnology (New York, N.Y.) · 2016Article
- Article
- Introduction to a special issue in memory of Paul S. Gross.Marine biotechnology (New York, N.Y.) · 2011Article
- Construction and characterization of two bacterial artificial chromosome libraries of grass carp.Marine biotechnology (New York, N.Y.) · 2010Article
- Construction and characterization of a bacterial artificial chromosome (BAC) library of Pacific white shrimp, Litopenaeus vannamei.Marine biotechnology (New York, N.Y.) · 2010Article
- Article
- MytiBase: a knowledgebase of mussel (M. galloprovincialis) transcribed sequences.BMC genomics · 2009Article
- Generation and analysis of ESTs from the eastern oyster, Crassostrea virginica Gmelin and identification of microsatellite and SNP markers.BMC genomics · 2007Article
- Fosmid library construction and initial analysis of end sequences in Zhikong scallop (Chlamys farreri).Marine biotechnology (New York, N.Y.)Article
- Construction and characterization of two bacterial artificial chromosome libraries of Zhikong scallop, Chlamys farreri Jones et Preston, and identification of BAC clones containing the genes involved in its innate immune system.Marine biotechnology (New York, N.Y.)Article
- Characterization of a BAC library from channel catfish Ictalurus punctatus: indications of high levels of chromosomal reshuffling among teleost genomes.Marine biotechnology (New York, N.Y.)Article
- A cDNA microarray for Crassostrea virginica and C. gigas.Marine biotechnology (New York, N.Y.)Article
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12 authors.
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Abstract
Large-insert genomic bacterial artificial chromosome (BAC) libraries of two culturally and economically important oyster species, Crassostrea virginica and C. gigas, have been developed as part of an international effort to develop tools and reagents that will advance our ability to conduct genetic and genomic research. A total of 73,728 C. gigas clones with an average insert size of 152 kb were picked and arrayed representing an 11.8-fold genome coverage. A total of 55,296 clones with an average insert size of 150 kb were picked and arrayed for C. virginica, also representing an 11.8-fold genome coverage. The C. gigas and C. virginica libraries were screened with probes derived from selected oyster genes using high-density BAC colony filter arrays. The probes identified 4 to 25 clones per gene for C. virginica and 5 to 50 clones per gene for C. gigas. We conducted a preliminary analysis of genetic polymorphism represented in the C. gigas library. The results suggest that the degree of divergence among similar sequences is highly variable and concentrated in intronic regions. Evidence supporting allelic polymorphism is reported for two genes and allelic and/or locus specific polymorphism for several others. Classical inheritance studies are needed to confirm the nature of these polymorphisms. The oyster BAC libraries are publicly available to the research community on a cost-recovery basis at (www.genome.clemson.edu).
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