ArticleThe American journal of tropical medicine and hygiene2006
Detection sensitivity and quantitation of Plasmodium falciparum var gene transcripts by real-time RT-PCR in comparison with conventional RT-PCR.
Article in The American journal of tropical medicine and hygiene, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed.
- In silico guided reconstruction and analysis of ICAM-1-binding var genes from Plasmodium falciparum.Scientific reports · 2018Article
- Differential PfEMP1 expression is associated with cerebral malaria pathology.PLoS pathogens · 2014Article
- The role of N-terminus of Plasmodium falciparum ORC1 in telomeric localization and var gene silencing.Nucleic acids research · 2012Article
- Variation among Plasmodium falciparum strains in their reliance on mitochondrial electron transport chain function.Eukaryotic cell · 2011Article
- Embryonic toxin expression in the cone snail Conus victoriae: primed to kill or divergent function?The Journal of biological chemistry · 2011Article
- Article
- Antimalarial exposure delays Plasmodium falciparum intra-erythrocytic cycle and drives drug transporter genes expression.PloS one · 2010Article
- Var transcription profiling of Plasmodium falciparum 3D7: assignment of cytoadherent phenotypes to dominant transcripts.Malaria journal · 2008Article
- Differential changes in Plasmodium falciparum var transcription during adaptation to culture.The Journal of infectious diseases · 2007Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Antigenic variation in Plasmodium falciparum erythrocyte membrane protein 1, caused by a switch in transcription of the encoding var gene, is an important feature of malaria. In this study, we quantified the relative abundance of var gene transcripts present in P. falciparum parasite clones using real-time reverse transcription-polymerase chain reaction (RT-PCR) and conventional RT-PCR combined with cloning and sequencing, with the aim of directly comparing the results obtained. When there was sufficient abundance of RNA for the real-time RT-PCR assay to be operating within the region of good reproducibility, RT-PCR and real-time RT-PCR tended to identify the same dominant transcript, although some transcript-specific issues were identified. When there were differences in the estimated relative amounts of minor transcripts, the RT-PCR assay tended to produce higher estimates than real-time RT-PCR. These results provide valuable information comparing RT-PCR and real-time RT-PCR analysis of samples with small quantities of RNA as might be expected in the analysis of field or clinical samples.
Indexed as
Identifiers
16896121PMC1601947What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.