ArticleThe Journal of cell biology2006
PKCalpha: a versatile key for decoding the cellular calcium toolkit.
Article in The Journal of cell biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 64 citations in OpenAlex.
- A high-performance genetically encoded sensor for cellular imaging of PKC activity in vivo.Nature communications · 2025Article
- Connexin 25 maintains self-renewal and functions of airway basal cells for airway regeneration.Stem cell research & therapy · 2024Article
- Decoding the Bell-Shaped Calcium Spikes in Phosphorylation Cycles of Flagella.International journal of molecular sciences · 2022Article
- Striking a balance: PIPExploration of neuroprotective therapy · 2021Article
- Dietary fructose enhances angiotensin II-stimulated NaAmerican journal of physiology. Renal physiology · 2020Article
- Combined Menin and EGFR Inhibitors Synergize to Suppress Colorectal Cancer via EGFR-Independent and Calcium-Mediated Repression of SKP2 Transcription.Cancer research · 2019Article
- Article
- PKCα diffusion and translocation are independent of an intact cytoskeleton.Scientific reports · 2017Article
- C2-domain mediated nano-cluster formation increases calcium signaling efficiency.Scientific reports · 2016Article
- TRPM2 regulates TXNIP-mediated NLRP3 inflammasome activation via interaction with p47 phox under high glucose in human monocytic cells.Scientific reports · 2016Article
- Photoswitchable diacylglycerols enable optical control of protein kinase C.Nature chemical biology · 2016Article
- Article
- Targeted activation of conventional and novel protein kinases C through differential translocation patterns.Molecular and cellular biology · 2014Article
- Mitotic lamin disassembly is triggered by lipid-mediated signaling.The Journal of cell biology · 2012Article
- Intercellular Ca(2+) waves: mechanisms and function.Physiological reviews · 2012Review
- Protein kinase C: the "masters" of calcium and lipid.Cold Spring Harbor perspectives in biology · 2011Review
- Rational method in the repetitive calcium oscillation measurement in wild type human epithelial kidney cells.Cytotechnology · 2011Article
- Ca2+ influx and protein scaffolding via TRPC3 sustain PKCbeta and ERK activation in B cells.Journal of cell science · 2010Article
- The annexins: spatial and temporal coordination of signaling events during cellular stress.Cellular and molecular life sciences : CMLS · 2009Review
- Lipid activation of protein kinases.Journal of lipid research · 2009Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conventional protein kinases C (cPKCs) play an essential role in signal transduction and are believed to integrate both global Ca(2+) transients and diacylglycerol signals. We provide evidence that PKCalpha is a ubiquitous readout sensor for the cellular Ca(2+) toolkit, including highly restricted elementary Ca(2+) release. Threshold stimulations of cells with Ca(2+)-mobilizing agonists resulted in PKCalpha translocation events with limited spatial spreads (<4 microm) comprising two groups of lifetimes; brief events (400-1,500 ms) exclusively mediated by Ca(2+)-C2 domain membrane interactions and long-lasting events (>4 s) resulting from longer DAG-C1a domain-mediated membrane interactions. Although upon uncaging NP-EGTA, which is a caged Ca(2+) compound, WT-PKCalpha displayed rapid membrane translocations within <250 ms, PKCalpha constructs with C2 domains mutated in their Ca(2+)-binding region lacked any Ca(2+)-dependent translocation. Flash photolysis of diazo-2, a photosensitive caged Ca(2+) buffer, revealed a biphasic membrane dissociation (slow and fast period) of WT-PKCalpha. The slow phase was absent in cells expressing PKCalpha-constructs containing mutated C1a-domains with largely reduced DAG binding. Thus, two groups of PKCalpha membrane interactions coexist; C2- and C1a-mediated interactions with different lifetimes but rapid interconversion. We conclude that PKCalpha can readout very fast and, spatially and temporally, very complex cellular Ca(2+) signals. Therefore, cPKCs are important transducers for the ubiquitous cellular Ca(2+) signaling toolkit.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.