ArticleNucleic acids research2006
An RNA aptamer that interferes with the DNA binding of the HSF transcription activator.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- An RNA aptamer to HP1/Swi6 facilitates heterochromatin formation at an ectopic locus in S.pombe.RNA biology · 2019Article
- HSF1 as a Cancer Biomarker and Therapeutic Target.Current cancer drug targets · 2019Review
- Aptamers: Uptake mechanisms and intracellular applications.Advanced drug delivery reviews · 2018Review
- RNA aptamers targeted for human αA-crystallin do not bind αB-crystallin, and spare the α-crystallin domain.Biochemical and biophysical research communications · 2017Article
- Upregulation of HSF1 in estrogen receptor positive breast cancer.Oncotarget · 2016Article
- Directing cellular information flow via CRISPR signal conductors.Nature methods · 2016Article
- Anti-Transcription Factor RNA Aptamers as Potential Therapeutics.Nucleic acid therapeutics · 2016Review
- RNA aptamer inhibitors of a restriction endonuclease.Nucleic acids research · 2015Article
- An RNA aptamer specific to Hsp70-ATP conformation inhibits its ATPase activity independent of Hsp40.Nucleic acid therapeutics · 2015Article
- Molecular and Functional Characterization of ssDNA Aptamers that Specifically Bind Leishmania infantum PABP.PloS one · 2015Article
- Inhibition of Rift Valley fever virus replication and perturbation of nucleocapsid-RNA interactions by suramin.Antimicrobial agents and chemotherapy · 2014Article
- In search of novel drug target sites on estrogen receptors using RNA aptamers.Nucleic acid therapeutics · 2014Article
- Selection and characterization of a DNA aptamer that can discriminate between cJun/cJun and cJun/cFos.PloS one · 2014Article
- Article
- A systematic study of the features critical for designing a high avidity multivalent aptamer.Nucleic acid therapeutics · 2013Article
- Multiplexed microcolumn-based process for efficient selection of RNA aptamers.Analytical chemistry · 2013Article
- Non-coding RNAs: key regulators of mammalian transcription.Trends in biochemical sciences · 2012Review
- An RNA aptamer perturbs heat shock transcription factor activity in Drosophila melanogaster.Nucleic acids research · 2011Article
- Knocking down gene function with an RNA aptamer expressed as part of an intron.Nucleic acids research · 2010Article
- An RNA-based transcription activator derived from an inhibitory aptamer.Nucleic acids research · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Heat shock factor (HSF) is a conserved and highly potent transcription activator. It is involved in a wide variety of important biological processes including the stress response and specific steps in normal development. Reagents that interfere with HSF function would be useful for both basic studies and practical applications. We selected an RNA aptamer that binds to HSF with high specificity. Deletion analysis defined the minimal binding motif of this aptamer to be two stems and one stem-loop joined by a three-way junction. This RNA aptamer interferes with normal interaction of HSF with its DNA element, which is a key regulatory step for HSF function. The DNA-binding domain plus a flanking linker region on the HSF (DL) is essential for the RNA binding. Additionally, this aptamer inhibits HSF-induced transcription in vitro in the complex milieu of a whole cell extract. In contrast to the previously characterized NF-kappaB aptamer, the HSF aptamer does not simply mimic DNA binding, but rather binds to HSF in a manner distinct from DNA binding to HSF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.