ArticleNucleic acids research2006
Engineering a high-affinity methyl-CpG-binding protein.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed.
- A scalable Tn5-based method for genome-wide DNA methylation profiling in development and disease.Nature communications · 2026Article
- Hybridization-based CpG methylation level detection using methyl-CpG-binding domain-fused luciferase.Analytical and bioanalytical chemistry · 2023Article
- Levels of base excision repair proteins in CD4Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2022Article
- Development and application of novel BiFC probes for cell sorting based on epigenetic modification.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2022Article
- Lead exposure induces dysregulation of constitutive heterochromatin hallmarks in live cells.Current research in toxicology · 2022Article
- Methylation multiplicity and its clinical values in cancer.Expert reviews in molecular medicine · 2021Review
- Avidity observed between a bivalent inhibitor and an enzyme monomer with a single active site.PloS one · 2021Article
- A Practical Guide to the Measurement and Analysis of DNA Methylation.American journal of respiratory cell and molecular biology · 2019Article
- Analytical epigenetics: single-molecule optical detection of DNA and histone modifications.Current opinion in biotechnology · 2019Review
- Analysis of DNA modifications in aging research.GeroScience · 2018Review
- Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97.Viruses · 2018Article
- Detection of methylation on dsDNA using nanopores in a MoSNanoscale · 2017Article
- High Resolution Imaging of DNA Methylation Dynamics using a Zebrafish Reporter.Scientific reports · 2017Article
- MethylMeter(®): bisulfite-free quantitative and sensitive DNA methylation profiling and mutation detection in FFPE samples.Epigenomics · 2016Article
- Characterization and directed evolution of a methyl-binding domain protein for high-sensitivity DNA methylation analysis.Protein engineering, design & selection : PEDS · 2015Article
- Dynamics of MBD2 deposition across methylated DNA regions during malignant transformation of human mammary epithelial cells.Nucleic acids research · 2015Article
- Single molecule and single cell epigenomics.Methods (San Diego, Calif.) · 2015Review
- Rapid microfluidic solid-phase extraction system for hyper-methylated DNA enrichment and epigenetic analysis.Biomicrofluidics · 2014Article
- Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein.The Analyst · 2014Article
- Heterochromatin dynamics during the differentiation process revealed by the DNA methylation reporter mouse, MethylRO.Stem cell reports · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Core members of the MBD protein family (MeCP2, MBD1, MBD2 and MBD4) share a methyl-CpG-binding domain that has a specific affinity for methylated CpG sites in double-stranded DNA. By multimerizing the MDB domain of Mbd1, we engineered a poly-MBD protein that displays methyl-CpG-specific binding in vitro with a dissociation constant that is >50-fold higher than that of a monomeric MBD. Poly-MBD proteins also localize to methylated foci in cells and can deliver a functional domain to reporter constructs in vivo. We propose that poly-MBD proteins are sensitive reagents for the detection of DNA methylation levels in isolated native DNA and for cytological detection of chromosomal CpG methylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.