ArticlePlant physiology2006
The frequency and efficiency of endogene suppression by transitive silencing signals is influenced by the length of sequence homology.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 29 citations in OpenAlex.
- MicroRNA-Induced Gene Silencing (MIGS): A Tool for Multi-Gene Silencing and Targeting Viruses in Plants.Plant biotechnology journal · 2026Article
- Multiple factors and features dictate the selective production of ct-siRNA in Arabidopsis.Communications biology · 2024Article
- Down-regulation of wheat Rubisco activase isoforms expression by virus-induced gene silencing.Plant direct · 2024Article
- Secondary siRNAs in Plants: Biosynthesis, Various Functions, and Applications in Virology.Frontiers in plant science · 2021Review
- The Whys and Wherefores of Transitivity in Plants.Frontiers in plant science · 2020Review
- RNAi-mediated endogene silencing in strawberry fruit: detection of primary and secondary siRNAs by deep sequencing.Plant biotechnology journal · 2017Article
- Analysis of the siRNA-Mediated Gene Silencing Process Targeting Three Homologous Genes Controlling Soybean Seed Oil Quality.PloS one · 2015Article
- Poplars with a PtDDM1-RNAi transgene have reduced DNA methylation and show aberrant post-dormancy morphology.Planta · 2013Article
- RNA-dependent RNA polymerase 6 is required for efficient hpRNA-induced gene silencing in plants.Molecules and cells · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transitivity, the spread of RNA silencing along primary target sequences, leads to the degradation of secondary targets that have no sequence homology to the initial silencing trigger. We demonstrate that increasing the distance between direct and adjacent target sequences in a transgenic primary target delays the onset of silencing of a secondary target gene. Silencing can spread in a 3' to 5' direction over a distance of at least 500 nucleotides (nt), but this requires consistently more time compared to a distance of 98 nt or 250 nt. The efficiency and frequency of transitive silencing of an endogene depends on the length of its sequence homology with the primary target. With a length of 500 nt, efficient silencing can eventually be established in all plants, whereas lengths of 250 nt and 98 nt homology result in less efficient and less frequent suppression. These results suggest that amplification of secondary small interfering RNAs (siRNAs) is a time-requiring process that gradually expands the population of siRNAs until a steady-state level is reached. Moreover, the length of the sequence homology in the primary target providing secondary siRNAs determines whether this steady-state level readily exceeds the threshold necessary for efficient silencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.