ArticlePlant physiology2006
Chimeric proteins suggest that the catalytic and/or C-terminal domains give CesA1 and CesA3 access to their specific sites in the cellulose synthase of primary walls.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 57 citations in OpenAlex.
- Essential amino acids in the Plant-Conserved and Class-Specific Regions of cellulose synthases.Plant physiology · 2023Article
- Transcriptome Analysis of Air Space-Type Variegation Formation inInternational journal of molecular sciences · 2022Article
- Gain-of-function mutation of AtDICE1, encoding a putative endoplasmic reticulum-localized membrane protein, causes defects in anisotropic cell elongation by disturbing cell wall integrity in Arabidopsis.Annals of botany · 2018Article
- The cell biology of secondary cell wall biosynthesis.Annals of botany · 2018Review
- Functional Analysis of Cellulose SynthaseFrontiers in plant science · 2018Article
- Functional Analysis of Cellulose Synthase (CESA) Protein Class Specificity.Plant physiology · 2017Article
- A complementation assay for in vivo protein structure/function analysis in Physcomitrella patens (Funariaceae).Applications in plant sciences · 2015Article
- The Arabidopsis cellulose synthase complex: a proposed hexamer of CESA trimers in an equimolar stoichiometry.The Plant cell · 2014Article
- Systems and synthetic biology approaches to alter plant cell walls and reduce biomass recalcitrance.Plant biotechnology journal · 2014Review
- The structure of the catalytic domain of a plant cellulose synthase and its assembly into dimers.The Plant cell · 2014Article
- Cellulose synthesis and its regulation.The arabidopsis book · 2014Article
- The anisotropy1 D604N mutation in the Arabidopsis cellulose synthase1 catalytic domain reduces cell wall crystallinity and the velocity of cellulose synthase complexes.Plant physiology · 2013Article
- Cracking the elusive alignment hypothesis: the microtubule-cellulose synthase nexus unraveled.Trends in plant science · 2012Review
- Complexes with mixed primary and secondary cellulose synthases are functional in Arabidopsis plants.Plant physiology · 2012Article
- Cellulose microfibril crystallinity is reduced by mutating C-terminal transmembrane region residues CESA1A903V and CESA3T942I of cellulose synthase.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- POM-POM2/cellulose synthase interacting1 is essential for the functional association of cellulose synthase and microtubules in Arabidopsis.The Plant cell · 2012Article
- Understanding Plant Cellulose Synthases through a Comprehensive Investigation of the Cellulose Synthase Family Sequences.Frontiers in plant science · 2011Article
- Update on mechanisms of plant cell wall biosynthesis: how plants make cellulose and other (1->4)-β-D-glycans.Plant physiology · 2011Review
- A missense mutation in the transmembrane domain of CESA4 affects protein abundance in the plasma membrane and results in abnormal cell wall biosynthesis in rice.Plant molecular biology · 2009Article
- Features of the primary wall CESA complex in wild type and cellulose-deficient mutants of Arabidopsis thaliana.Journal of experimental botany · 2008Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CesA1 and CesA3 are thought to occupy noninterchangeable sites in the cellulose synthase making primary wall cellulose in Arabidopsis (Arabidopsis thaliana L. Heynh). With domain swaps and deletions, we show that sites C terminal to transmembrane domain 2 give CesAs access to their individual sites and, from dominance and recessive behavior, deduce that certain CesA alleles exclude others from accessing each site. Constructs that swapped or deleted N-terminal domains were stably transformed into the wild type and into the temperature-sensitive mutants rsw1 (Ala-549Val in CesA1) and rsw5 (Pro-1056Ser in CesA3). Dominant-positive behavior was assayed as root elongation at the restrictive temperature and dominant-negative effects were observed at the permissive temperature. A protein with the catalytic and C-terminal domains of CesA1 and the N-terminal domain of CesA3 promoted growth only in rsw1 consistent with it accessing the CesA1 site even though it contained the CesA3 N-terminal domain. A protein having the CesA3 catalytic and C-terminal domains linked to the CesA1 N-terminal domain dramatically affected growth, but only in the CesA3 mutant. This is consistent with the operation of the same access rule taking this chimeric protein to the CesA3 site. In this case, however, the transgene behaved as a genotype-specific dominant negative, causing a 60% death rate in rsw5, but giving no visible phenotype in wild type or rsw1. We therefore hypothesize that possession of CesA3(WT) protects Columbia and rsw1 from the lethal effects of this chimeric protein, whereas the mutant protein (CesA3(rsw5)) does not.
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