Evidence map›Paper›PMID 16891503›Full record

ArticleJournal of clinical microbiology2006

Robust sequence selection method used to develop the FluChip diagnostic microarray for influenza virus.

Martin Mehlmann, Erica D Dawson, Michael B Townsend, James A Smagala, Chad L Moore, Catherine B Smith, Nancy J Cox, Robert D Kuchta, Kathy L Rowlen

Open access · greenAbstract read
In one paragraph

Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 39 citations in OpenAlex.

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  11. Genetic variation at a single locus and age of onset for Alzheimer's disease.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2010
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  16. Detection of adamantane-resistant influenza on a microarray.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2008
    Article
  17. Direct sequence detection of structured h5 influenza viral RNA.The Journal of molecular diagnostics : JMD · 2008
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Martin MehlmannDepartment of Chemistry and Biochemistry, University of Colorado, UCB215, Boulder, CO 80303, USA.
Erica D Dawson
Michael B Townsend
James A Smagala
Chad L Moore
Catherine B Smith
Nancy J Cox
Robert D Kuchta
Kathy L Rowlen
University of Colorado Boulder · USCenters for Disease Control and Prevention · US

Funding

Development of Diagnostic Microarray for Influenza AU01AI056528 · NIAID · UNIVERSITY OF COLORADO AT BOULDER · PI ROWLEN, KATHY L · 2003 to 2007
$2.0M
NIAID NIH HHS U01 AI056528NIAID NIH HHS U01 AI056528-03
6 · The paper itself

Abstract

DNA microarrays have proven to be powerful tools for gene expression analyses and are becoming increasingly attractive for diagnostic applications, e.g., for virus identification and subtyping. The selection of appropriate sequences for use on a microarray poses a challenge, particularly for highly mutable organisms such as influenza viruses, human immunodeficiency viruses, and hepatitis C viruses. The goal of this work was to develop an efficient method for mining large databases in order to identify regions of conservation in the influenza virus genome. From these regions of conservation, capture and label sequences capable of discriminating between different viral types and subtypes were selected. The salient features of the method were the use of phylogenetic trees for data reduction and the selection of a relatively small number of capture and label sequences capable of identifying a broad spectrum of influenza viruses. A detailed experimental evaluation of the selected sequences is described in a companion paper. The software is freely available under the General Public License at http://www.colorado.edu/chemistry/RGHP/software/.

Indexed as

Conserved SequenceGenome, ViralOligonucleotide Array Sequence AnalysisOligonucleotide ProbesComputational BiologyDatabases, Nucleic AcidInfluenza A virusInfluenza B virusPhylogenyRNA, ViralSoftwareOligonucleotide ProbesRNA, Viral

Identifiers

PMID16891503
PMCPMC1594657
OpenAlexW2116309501

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.