ArticleJournal of clinical microbiology2006
Enhanced reverse transcription-PCR assay for detection of norovirus genogroup I.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Hospital-based norovirus surveillance in children <5 years of age from 2017 to 2019 in India.Journal of medical virology · 2024Article
- Significance of norovirus in occupational health: a review of published norovirus outbreaks in Central and Northern Europe.International archives of occupational and environmental health · 2020Review
- Performance of the QIAstat-Dx Gastrointestinal Panel for Diagnosing Infectious Gastroenteritis.Journal of clinical microbiology · 2020Article
- Preparation of MS2 Phage-Like Particles and Their Use As Potential Process Control Viruses for Detection and Quantification of Enteric RNA Viruses in Different Matrices.Frontiers in microbiology · 2016Article
- Molecular Diagnostic Methods for Detection and Characterization of Human Noroviruses.The open microbiology journal · 2016Article
- Development of a Practical Method to Detect Noroviruses Contamination in Composite Meals.Food and environmental virology · 2015Article
- Norovirus.Clinical microbiology reviews · 2015Review
- Environmental detection of genogroup I, II, and IV noroviruses by using a generic real-time reverse transcription-PCR assay.Applied and environmental microbiology · 2013Article
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- Article
- Development of a latex agglutination test for norovirus detection.Journal of microbiology (Seoul, Korea) · 2010Article
- Validation of laboratory-developed molecular assays for infectious diseases.Clinical microbiology reviews · 2010Review
- Effects of technological processes on the tenacity and inactivation of norovirus genogroup II in experimentally contaminated foods.Applied and environmental microbiology · 2010Article
- An economical tandem multiplex real-time PCR technique for the detection of a comprehensive range of respiratory pathogens.Viruses · 2009Article
- The use of Armored RNA as a multi-purpose internal control for RT-PCR.Journal of virological methods · 2008Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a one-tube reverse transcription (RT)-PCR method using the real-time TaqMan PCR system for the detection of norovirus genogroup I (NV GGI). By introduction of a novel probe based on locked nucleic acid technology, we enhanced the sensitivity of the assay compared to those of conventional TaqMan probes. The sensitivity of the NV GGI RT-PCR was determined by probit analysis with defined RNA standards and quantified norovirus isolates to 711 copies/ml (95% detection limit). In order to detect PCR inhibition, we included a heterologous internal control (IC) system based on phage MS2. This internally controlled RT-PCR was tested on different real-time PCR platforms, LightCycler, Rotorgene, Mastercycler EP realplex, and ABI Prism. Compared to the assay without an IC, the duplex RT-PCR exhibited no reduction in sensitivity in clinical samples. In combination with an established NV GGII real-time RT-PCR, we used the novel assay in a routine assay for diagnosis of clinical and food-borne norovirus infection. We applied this novel assay to analyze outbreaks of nonbacterial acute gastroenteritis. Norovirus of GGI was detected in these outbreaks. Sequence and similarity plot analysis of open reading frame 1 (ORF1) and ORF2 showed two genotypes, GGI/2 and GGI/4, in semiclosed communities.
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