ArticleJournal of clinical microbiology2006
Multicenter comparison of nucleic acid extraction methods for detection of severe acute respiratory syndrome coronavirus RNA in stool specimens.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
- Article
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- The COVID-19 pandemic and its implications on the environment.Environmental research · 2021Review
- An optimized and robust PEG precipitation method for detection of SARS-CoV-2 in wastewater.The Science of the total environment · 2021Article
- SARS-CoV, MERS-CoV and SARS-CoV-2: A Diagnostic Challenge.Measurement : journal of the International Measurement Confederation · 2021Article
- Making Waves: Coronavirus detection, presence and persistence in the water environment: State of the art and knowledge needs for public health.Water research · 2020Review
- COVID-19: molecular diagnostics overview.Journal of molecular medicine (Berlin, Germany) · 2020Review
- Performance and workflow assessment of six nucleic acid extraction technologies for use in resource limited settings.PloS one · 2019Article
- Performance of a commercial assay for detecting and quantifying HEV RNA in faeces.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2018Article
- Evaluation of Methods for the Concentration and Extraction of Viruses from Sewage in the Context of Metagenomic Sequencing.PloS one · 2017Article
- A Novel Sample Processing Method for Rapid Detection of Tuberculosis in the Stool of Pediatric Patients Using the Xpert MTB/RIF Assay.PloS one · 2016Article
- Detection of small amounts of human adenoviruses in stools: comparison of a new immuno real-time PCR assay with classical tools.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2014Article
- High-throughput direct fecal PCR assay for detection of Mycobacterium avium subsp. paratuberculosis in sheep and cattle.Journal of clinical microbiology · 2014Article
- Comparative evaluation of commercially available manual and automated nucleic acid extraction methods for rotavirus RNA detection in stools.Journal of virological methods · 2013Article
- Performance evaluation of the Maxwell 16 System for extraction of influenza virus RNA from diverse samples.PloS one · 2012Article
- Article
- Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example.Virology journal · 2011Article
- MagaZorb: a simple tool for rapid isolation of viral nucleic acids.The Journal of infectious diseases · 2010Article
- Multicenter comparative evaluation of five commercial methods for toxoplasma DNA extraction from amniotic fluid.Journal of clinical microbiology · 2009Article
- Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor.Biosensors & bioelectronics · 2009Article
Corrections and comments
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Authors and funding
20 authors at 8 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The emergence of a novel coronavirus (CoV) as the cause of severe acute respiratory syndrome (SARS) catalyzed the development of rapid diagnostic tests. Stool samples have been shown to be appropriate for diagnostic testing for SARS CoV, although it has been recognized to be a heterogeneous and difficult sample that contains amplification inhibitors. Limited information on the efficiency of extraction methods for the purification and concentration of SARS CoV RNA from stool samples is available. Our study objectives were to determine the optimal extraction method for SARS CoV RNA detection and to examine the effect of increased specimen volume for the detection of SARS CoV RNA in stool specimens. We conducted a multicenter evaluation of four automated and four manual extraction methods using dilutions of viral lysate in replicate mock stool samples, followed by quantitation of SARS CoV RNA using real-time reverse transcriptase PCR. The sensitivities of the manual methods ranged from 50% to 100%, with the Cortex Biochem Magazorb method, a magnetic bead isolation method, allowing detection of all 12 positive samples. The sensitivities of the automated methods ranged from 75% to 100%. The bioMérieux NucliSens automated extractor and miniMag extraction methods each had a sensitivity of 100%. Examination of the copy numbers detected and the generation of 10-fold dilutions of the extracted material indicated that a number of extraction methods retained inhibitory substances that prevented optimal amplification. Increasing the volume of sample input did improve detection. This information could be useful for the extraction of other RNA viruses from stool samples and demonstrates the need to evaluate extraction methods for different specimen types.
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