ArticleBiophysical journal2006
Localization of the pH gate in Kir1.1 channels.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 23 citations in OpenAlex.
- Impact of aldosterone deficiency on the development of diuretic resistance in mice.Pflugers Archiv : European journal of physiology · 2025Article
- LRET Determination of Molecular Distances during pH Gating of the Mammalian Inward Rectifier Kir1.1b.Biophysical journal · 2018Article
- State-dependent network connectivity determines gating in a K+ channel.Structure (London, England : 1993) · 2014Article
- Lack of negatively charged residues at the external mouth of Kir2.2 channels enable the voltage-dependent block by external Mg2+.PloS one · 2014Article
- Cxs and Panx- hemichannels in peripheral and central chemosensing in mammals.Frontiers in cellular neuroscience · 2014Review
- Inhibition of ROMK channels by low extracellular K+ and oxidative stress.American journal of physiology. Renal physiology · 2013Article
- Residues at the outer mouth of Kir1.1 determine K-dependent gating.Biophysical journal · 2012Article
- Contribution of residues in second transmembrane domain of ASIC1a protein to ion selectivity.The Journal of biological chemistry · 2012Article
- Potassium-dependent activation of Kir4.2 K⁺ channels.The Journal of physiology · 2011Article
- The pore structure and gating mechanism of K2P channels.The EMBO journal · 2011Article
- Modulation of Kir1.1 inactivation by extracellular Ca and Mg.Biophysical journal · 2011Article
- Regulation and function of potassium channels in aldosterone-sensitive distal nephron.Current opinion in nephrology and hypertension · 2010Review
- The ClC-3 Cl-/H+ antiporter becomes uncoupled at low extracellular pH.The Journal of biological chemistry · 2010Article
- An intersubunit salt bridge near the selectivity filter stabilizes the active state of Kir1.1.Biophysical journal · 2009Article
- Extracellular proton-modulated pore-blocking effect of the anticonvulsant felbamate on NMDA channels.Biophysical journal · 2007Article
- Crystal structure of a Kir3.1-prokaryotic Kir channel chimera.The EMBO journal · 2007Article
- External K activation of Kir1.1 depends on the pH gate.Biophysical journal · 2007Article
- Random mutagenesis screening indicates the absence of a separate H(+)-sensor in the pH-sensitive Kir channels.Channels (Austin, Tex.)Article
- Kir4.1 K+ channels are regulated by external cations.Channels (Austin, Tex.)Article
- The Kir channel immunoglobulin domain is essential for Kir1.1 (ROMK) thermodynamic stability, trafficking and gating.Channels (Austin, Tex.)Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
We used cysteine-modifying reagents to localize the pH-sensitive gate in the renal inward-rectifier K(+) channel Kir1.1a (ROMK1). Cytoplasmic-side methanethiosulfonate (MTS) reagents blocked K(+) permeation in native Kir1.1 channels, expressed in Xenopus oocytes. Replacement of three cysteines in the N-terminus, C-terminus, and transmembrane domains eliminated this sensitivity to MTS reagents, as measured with inside-out macropatches. Reintroduction of one cysteine at 175-Kir1.1a in the second transmembrane domain allowed blockade of the open channel by the MTS reagents MTSEA, MTSET, and MTSES and by Ag(+). However, closure of the channel by low pH protected it from modification. Cysteine was also introduced into position G223, which is thought to line the cytoplasmic pore of the channel. MTSET blocked G223C in both the open and closed state. In contrast, MTSEA reduced G223C single-channel conductance from 40 to 23 pS but did not produce complete block. We conclude that cytoplasmic acidification induces a conformational change in the channel protein that prevents access of cysteine-modifying reagents, and presumably also K(+) ions, to the transmembrane pore from the cytoplasm. This is consistent with localization of the Kir1.1 pH gate at the helix bundle crossing near the cytoplasmic end of the transmembrane pore.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.