ArticleBiophysical journal2006
Rheology of passive and adhesion-activated neutrophils probed by atomic force microscopy.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Frequency-dependent cellular microrheology with pyramidal atomic force microscopy probes.Biophysical journal · 2026Article
- Microrheology: From Video Microscopy to Optical Tweezers.Micromachines · 2025Review
- Controlling fluid flow rate to separate leukocytes and cancer cells based on stiffness differences.Mikrochimica acta · 2025Article
- Review
- Quantifying both viscoelasticity and surface tension: Why sharp tips overestimate cell stiffness.Biophysical journal · 2024Article
- Neutrophil biology in injuries and diseases of the central and peripheral nervous systems.Progress in neurobiology · 2023Review
- Changes in the mechanical properties of human mesenchymal stem cells during differentiation.Royal Society open science · 2023Article
- Ceragenin CSA-13 displays high antibacterial efficiency in a mouse model of urinary tract infection.Scientific reports · 2022Article
- Engineering physical microenvironments to study innate immune cell biophysics.APL bioengineering · 2022Review
- Baseline neutrophil-lymphocyte ratio can be associated with hematoma expansion in patients with intracerebral hemorrhage: a retrospective observational study.BMC neuroscience · 2022Observational
- Voltage-Driven Alterations to Neuron Viscoelasticity.Bioelectricity · 2022Article
- EMT changes actin cortex rheology in a cell-cycle-dependent manner.Biophysical journal · 2021Article
- The force loading rate drives cell mechanosensing through both reinforcement and cytoskeletal softening.Nature communications · 2021Article
- Rapid viscoelastic changes are a hallmark of early leukocyte activation.Biophysical journal · 2021Article
- Real-time deformability cytometry reveals sequential contraction and expansion during neutrophil priming.Journal of leukocyte biology · 2019Article
- Inhibition of inflammatory response in human keratinocytes by magnetic nanoparticles functionalized with PBP10 peptide derived from the PIP2-binding site of human plasma gelsolin.Journal of nanobiotechnology · 2019Article
- Neutrophils in traumatic brain injury (TBI): friend or foe?Journal of neuroinflammation · 2018Review
- AFM and Microrheology in the Zebrafish Embryo Yolk Cell.Journal of visualized experiments : JoVE · 2017Article
- Mechanical deformation induces depolarization of neutrophils.Science advances · 2017Article
- Unbiased High-Precision Cell Mechanical Measurements with Microconstrictions.Biophysical journal · 2017Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rheology of neutrophils in their passive and activated states plays a key role in determining their function in response to inflammatory stimuli. Atomic force microscopy was used to study neutrophil rheology by measuring the complex shear modulus G*(omega) of passive nonadhered rat neutrophils on poly(HEMA) and neutrophils activated through adhesion to glass. G*(omega) was measured over three frequency decades (0.1-102.4 Hz) by indenting the cells 500 nm with a spherical tip and then applying a 50-nm amplitude multi-frequency signal. G*(omega) of both passive and adhered neutrophils increased as a power law with frequency, with a coupling between elastic (G') and loss (G'') moduli. For passive neutrophils at 1.6 Hz, G' = 380 +/- 121 Pa, whereas G'' was fourfold smaller and the power law coefficient was of x = 1.184. Adhered neutrophils were over twofold stiffer with a lower slope (x = 1.148). This behavior was adequately described by the power law structural damping model but not by liquid droplet and Kelvin models. The increase in stiffness with frequency may modulate neutrophil transit, arrest, and transmigration in vascular microcirculation.
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