ArticleBiophysical journal2006
Lateral mobility of membrane-binding proteins in living cells measured by total internal reflection fluorescence correlation spectroscopy.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 78 citations in OpenAlex.
- Mapping Protein Diffusion from the Plasma Membrane to the Nucleus: Insights from Fluorescence Correlation Spectroscopy.Biomolecules · 2026Review
- Direct visualization of native GSDMD pores reveals lipid-driven stabilization during pyroptosis.Science advances · 2026Article
- DHHC5 Mediates β-Adrenergic Signaling in Cardiomyocytes by Targeting Gα Proteins.Biophysical journal · 2020Article
- Site-Directed Fluorescence Approaches for Dynamic Structural Biology of Membrane Peptides and Proteins.Frontiers in molecular biosciences · 2019Review
- Quantifying Reversible Surface Binding via Surface-Integrated Fluorescence Correlation Spectroscopy.Nano letters · 2018Article
- Disruption of Ankyrin B and Caveolin-1 Interaction Sites Alters NaBiophysical journal · 2017Article
- An extended model of vesicle fusion at the plasma membrane to estimate protein lateral diffusion from TIRF microscopy images.BMC bioinformatics · 2017Article
- Surface Trafficking of APP and BACE in Live Cells.Traffic (Copenhagen, Denmark) · 2015Article
- Photosystem II antenna phosphorylation-dependent protein diffusion determined by fluorescence correlation spectroscopy.Scientific reports · 2013Article
- A spatial model for integrin clustering as a result of feedback between integrin activation and integrin binding.Biophysical journal · 2012Article
- Fluorescence correlation spectroscopy: past, present, future.Biophysical journal · 2011Review
- Comparison of protein behavior between wild-type and G601S hERG in living cells by fluorescence correlation spectroscopy.The journal of physiological sciences : JPS · 2011Article
- Measuring surface binding thermodynamics and kinetics by using total internal reflection with fluorescence correlation spectroscopy: practical considerations.The journal of physical chemistry. B · 2011Article
- Imaging with total internal reflection fluorescence microscopy for the cell biologist.Journal of cell science · 2010Review
- Direct observation and quantitative analysis of Lck exchange between plasma membrane and cytosol in living T cells.The Journal of biological chemistry · 2010Article
- Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes.Journal of structural biology · 2009Article
- Positive feedback regulation results in spatial clustering and fast spreading of active signaling molecules on a cell membrane.The Journal of chemical physics · 2009Article
- Vesicle diffusion close to a membrane: intermembrane interactions measured with fluorescence correlation spectroscopy.Biophysical journal · 2008Article
- Total internal reflection fluorescence correlation spectroscopy: effects of lateral diffusion and surface-generated fluorescence.Biophysical journal · 2008Article
- Supercritical angle fluorescence correlation spectroscopy.Biophysical journal · 2008Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Total internal reflection fluorescence correlation spectroscopy (TIR-FCS) allows us to measure diffusion constants and the number of fluorescent molecules in a small area of an evanescent field generated on the objective of a microscope. The application of TIR-FCS makes possible the characterization of reversible association and dissociation rates between fluorescent ligands and their receptors in supported phospholipid bilayers. Here, for the first time, we extend TIR-FCS to a cellular application for measuring the lateral diffusion of a membrane-binding fluorescent protein, farnesylated EGFP, on the plasma membranes of cultured HeLa and COS7 cells. We detected two kinds of diffusional motion-fast three-dimensional diffusion (D(1)) and much slower two-dimensional diffusion (D(2)), simultaneously. Conventional FCS and single-molecule tracking confirmed that D(1) was free diffusion of farnesylated EGFP close to the plasma membrane in cytosol and D(2) was lateral diffusion in the plasma membrane. These results suggest that TIR-FCS is a powerful technique to monitor movement of membrane-localized molecules and membrane dynamics in living cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.