ArticleThe EMBO journal2006
Genome-wide replication profiles of S-phase checkpoint mutants reveal fragile sites in yeast.
Article in The EMBO journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 72 citations in OpenAlex.
- 3D confinement physically regulates cell cycle progression in budding yeast.Nature communications · 2026Article
- The increase in cell death rates in caloric restricted cells of the yeast helicase mutant rrm3 is Sir complex dependent.Scientific reports · 2023Article
- ATR protects ongoing and newly assembled DNA replication forks through distinct mechanisms.Cell reports · 2023Article
- Smc5/6 functions with Sgs1-Top3-Rmi1 to complete chromosome replication at natural pause sites.Nature communications · 2021Article
- Small RNAs, Big Diseases.International journal of molecular sciences · 2020Review
- Role of PCNA and RFC in promoting Mus81-complex activity.BMC biology · 2017Article
- Environmental change drives accelerated adaptation through stimulated copy number variation.PLoS biology · 2017Article
- Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation.Nucleic acids research · 2017Article
- Absence of Non-histone Protein Complexes at Natural Chromosomal Pause Sites Results in Reduced Replication Pausing in Aging Yeast Cells.Cell reports · 2016Article
- Recruitment of Mcm10 to Sites of Replication Initiation Requires Direct Binding to the Minichromosome Maintenance (MCM) Complex.The Journal of biological chemistry · 2016Article
- Review
- Replication initiation and genome instability: a crossroads for DNA and RNA synthesis.Cellular and molecular life sciences : CMLS · 2014Review
- Are common fragile sites merely structural domains or highly organized "functional" units susceptible to oncogenic stress?Cellular and molecular life sciences : CMLS · 2014Review
- MCM10: one tool for all-Integrity, maintenance and damage control.Seminars in cell & developmental biology · 2014Review
- Genome-wide high-resolution mapping of chromosome fragile sites in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Mcm10 deficiency causes defective-replisome-induced mutagenesis and a dependency on error-free postreplicative repair.Cell cycle (Georgetown, Tex.) · 2014Article
- RNF4 and PLK1 are required for replication fork collapse in ATR-deficient cells.Genes & development · 2013Article
- Enigmatic roles of Mcm10 in DNA replication.Trends in biochemical sciences · 2013Review
- Four pillars of the S-phase checkpoint.Genes & development · 2013Article
- Rev3, the catalytic subunit of Polζ, is required for maintaining fragile site stability in human cells.Nucleic acids research · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The S-phase checkpoint kinases Mec1 and Rad53 in the budding yeast, Saccharomyces cerevisiae, are activated in response to replication stress that induces replication fork arrest. In the absence of a functional S-phase checkpoint, stalled replication forks collapse and give rise to chromosome breakage. In an attempt to better understand replication dynamics in S-phase checkpoint mutants, we developed a replication origin array for budding yeast that contains 424 of 432 previously identified potential origin regions. As expected, mec1-1 and rad53-1 mutants failed to inhibit late origin activation. Surprisingly however, 17 early-firing regions were not replicated efficiently in these mutants. This was not due to a lack of initiation, but rather to problems during elongation, as replication forks arrested in close proximity to these origins, resulting in the accumulation of small replication intermediates and eventual replication fork collapse. Importantly, these regions were not only prone to chromosome breakage in the presence of exogenous stress but also in its absence, similar to fragile sites in the human genome.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.