ArticleThe Biochemical journal2006
Spatial segregation of transport and signalling functions between human endothelial caveolae and lipid raft proteomes.
Article in The Biochemical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 73 citations in OpenAlex.
- Intracellular Membrane Transport in Vascular Endothelial Cells.International journal of molecular sciences · 2023Review
- Routing Nanomolar Protein Cargoes to Lipid Raft-Mediated/Caveolar Endocytosis through a Ganglioside GM1-Specific Recognition Tag.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- The Epstein-Barr virus LMP1 interactome: biological implications and therapeutic targets.Future virology · 2018Article
- Caveolae provide a specialized membrane environment for respiratory syncytial virus assembly.Journal of cell science · 2017Article
- Assessment of electrophile damage in a human brain endothelial cell line utilizing a clickable alkyne analog of 2-chlorohexadecanal.Free radical biology & medicine · 2016Article
- Article
- Multiple sphingolipid abnormalities following cerebral microendothelial hypoxia.Journal of neurochemistry · 2014Article
- A central role for vimentin in regulating repair function during healing of the lens epithelium.Molecular biology of the cell · 2014Article
- Epstein-Barr virus LMP1 modulates lipid raft microdomains and the vimentin cytoskeleton for signal transduction and transformation.Journal of virology · 2013Article
- Facing glycosphingolipid-Shiga toxin interaction: dire straits for endothelial cells of the human vasculature.Cellular and molecular life sciences : CMLS · 2013Review
- Targeting receptor-mediated endocytotic pathways with nanoparticles: rationale and advances.Advanced drug delivery reviews · 2013Review
- Tight junctions at the blood brain barrier: physiological architecture and disease-associated dysregulation.Fluids and barriers of the CNS · 2012Article
- Endothelial membrane reorganization during leukocyte extravasation.Cellular and molecular life sciences : CMLS · 2012Review
- Remodelling of membrane rafts expression in lung cells as an early sign of mechanotransduction-signalling in pulmonary edema.Journal of lipids · 2011Article
- Venous and arterial endothelial proteomics: mining for markers and mechanisms of endothelial diversity.Expert review of proteomics · 2010Review
- BASP1 promotes apoptosis in diabetic nephropathy.Journal of the American Society of Nephrology : JASN · 2010Article
- Functions of lipid raft membrane microdomains at the blood-brain barrier.Journal of molecular medicine (Berlin, Germany) · 2009Review
- The effect of a spatially heterogeneous transmural water flux on concentration polarization of low density lipoprotein in arteries.Biophysical journal · 2009Article
- Distinct regions within the erlins are required for oligomerization and association with high molecular weight complexes.The Journal of biological chemistry · 2009Article
- Formation and function of ceramide-enriched membrane platforms with CD38 during M1-receptor stimulation in bovine coronary arterial myocytes.American journal of physiology. Heart and circulatory physiology · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipid rafts and caveolae are biochemically similar, specialized domains of the PM (plasma membrane) that cluster specific proteins. However, they are morphologically distinct, implying different, possibly complementary functions. Two-dimensional gel electrophoresis preceding identification of proteins by MS was used to compare the relative abundance of proteins in DRMs (detergent-resistant membranes) isolated from HUVEC (human umbilical-vein endothelial cells), and caveolae immunopurified from DRM fractions. Various signalling and transport proteins were identified and additional cell-surface biotinylation revealed the majority to be exposed, demonstrating their presence at the PM. In resting endothelial cells, the scaffold of immunoisolated caveolae consists of only few resident proteins, related to structure [CAV1 (caveolin-1), vimentin] and transport (V-ATPase), as well as the GPI (glycosylphosphatidylinositol)-linked, surface-exposed protein CD59. Further quantitative characterization by immunoblotting and confocal microscopy of well-known [eNOS (endothelial nitric oxide synthase) and CAV1], less known [SNAP-23 (23 kDa synaptosome-associated protein) and BASP1 (brain acid soluble protein 1)] and novel [C8ORF2 (chromosome 8 open reading frame 2)] proteins showed different subcellular distributions with none of these proteins being exclusive to either caveolae or DRM. However, the DRM-associated fraction of the novel protein C8ORF2 (approximately 5% of total protein) associated with immunoseparated caveolae, in contrast with the raft protein SNAP-23. The segregation of caveolae from lipid rafts was visually confirmed in proliferating cells, where CAV1 was spatially separated from eNOS, SNAP-23 and BASP1. These results provide direct evidence for the previously suggested segregation of transport and signalling functions between specialized domains of the endothelial plasma membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.